Development of an immunosensor for the detection of vitellogenin using impedance spectroscopy

Darain, Farzana, Park, Deog-Su, Park, Jang-Su and Shim, Yoon-Bo (2004) Development of an immunosensor for the detection of vitellogenin using impedance spectroscopy. Biosensors and Bioelectronics, 19 10: 1245-1252. doi:10.1016/j.bios.2003.11.014


Author Darain, Farzana
Park, Deog-Su
Park, Jang-Su
Shim, Yoon-Bo
Title Development of an immunosensor for the detection of vitellogenin using impedance spectroscopy
Journal name Biosensors and Bioelectronics   Check publisher's open access policy
ISSN 0956-5663
1873-4235
Publication date 2004-05-15
Year available 2003
Sub-type Article (original research)
DOI 10.1016/j.bios.2003.11.014
Volume 19
Issue 10
Start page 1245
End page 1252
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
A simple and direct immunosensor for the determination of carp (Carassius auratus) vitellogenin (Vtg), a female-specific protein, has been proposed based on an antibody-captured conducting polymer-coated electrode. The monoclonal antibody specific to carp (C. auratus) Vtg was immobilized by covalent coupling to the carboxylic acid group on the polymer. The antibody immobilization and antibody–antigen interaction have been demonstrated by means of quartz crystal microbalance and impedance spectroscopic techniques. The impedance change occurred at the sensor surface due to the specific immuno-interaction was utilized to determine Vtg. The sensor showed high selectivity and sensitive response to Vtg in a buffered medium without redox probe. Vtg was determined in the linear range from 1.0 to 8.0 μg/l with the standard deviation of ±0.13 (n=3) and the detection limit was determined to be 0.42 μg/l. This method was applied to the determination of Vtg in real male and female carp (C. auratus) serum samples.
© 2003 Elsevier B.V. All rights reserved.
Keyword Vitellogenin
Immunosensor
Impedance
QCM
Conducting polymer
Reagentless system
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Available online 23 December 2003.

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Tue, 08 Feb 2011, 02:25:22 EST by Dr Farzana Darain on behalf of Aust Institute for Bioengineering & Nanotechnology