A separation-free amperometric immunosensor for vitellogenin based on screen-printed carbon arrays modified with a conductive polymer

Darain, Farzana, Park, Doeg Su, Park, Jang-Su, Chang, Seung-Cheol and Shim, Yoon-Bo (2005) A separation-free amperometric immunosensor for vitellogenin based on screen-printed carbon arrays modified with a conductive polymer. Biosensors & Bioelectronics, 20 9: 1780-1787.


Author Darain, Farzana
Park, Doeg Su
Park, Jang-Su
Chang, Seung-Cheol
Shim, Yoon-Bo
Title A separation-free amperometric immunosensor for vitellogenin based on screen-printed carbon arrays modified with a conductive polymer
Journal name Biosensors & Bioelectronics   Check publisher's open access policy
ISSN 0956-5663
1873-4235
Publication date 2005-03-15
Year available 2004
Sub-type Article (original research)
DOI 10.1016/j.bios.2004.07.006
Volume 20
Issue 9
Start page 1780
End page 1787
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract A disposable amperometric immunosensor was studied for the rapid detection of carp (Carassius auratus) Vitellogenin (Vtg). The sensor was fabricated based on screen-printed carbon arrays (SPCAs) containing eight carbon working and an integrated carbon counter electrodes. To construct the sensor, a conducting polymer (poly-terthiophene carboxylic acid) was electropolymerized on the surface of working electrodes and the polymer-coated SPCAs was characterized by SEM. Horseradish peroxidase (HRP) and a monoclonal antibody (anti-Vtg) specific to carp Vtg were covalently attached onto the polymer modified SPCAs. The immobilization of HRP and anti-Vtg onto the polymer-coated SPCAs was examined using cyclic voltammetry and quartz crystal microbalance studies. In order to detect the amount of Vtg, glucose oxidase (GOx)-labelled Vtg bound to the sensor surface under competition with the Vtg analyte was quantified amperometrically using glucose as a substrate. The performance of the eight sensors in arrays was evaluated by obtaining the calibration plots for Vtg. The sensor arrays exhibit a linear range of the Vtg concentration from 0.25 to 7.8 ng/ml and the detection limit was determined to be 0.09 ng/ml. Furthermore, the performance of the immunosensor for the determination of Vtg was evaluated by a standard addition method performed in fish serum samples.
© 2004 Elsevier B.V. All rights reserved.
Keyword Screen-printed carbon arrays
Vitellogenin
Conducting polymer
Amperometric immunosensor
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Available online 12 August 2004

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Mon, 07 Feb 2011, 16:22:12 EST by Dr Farzana Darain on behalf of Aust Institute for Bioengineering & Nanotechnology