Antibody immobilization onto polystyrene substrate - On-chip immunoassay for horse IgG based on fluorescence

Darain, Farzana, Gan, Kai Ling and Tjin, Swee Chuan (2009) Antibody immobilization onto polystyrene substrate - On-chip immunoassay for horse IgG based on fluorescence. Biomedical Microdevices, 11 3: 653-661. doi:10.1007/s10544-008-9275-3


Author Darain, Farzana
Gan, Kai Ling
Tjin, Swee Chuan
Title Antibody immobilization onto polystyrene substrate - On-chip immunoassay for horse IgG based on fluorescence
Journal name Biomedical Microdevices   Check publisher's open access policy
ISSN 1387-2176
1572-8781
Publication date 2009-06
Sub-type Article (original research)
DOI 10.1007/s10544-008-9275-3
Volume 11
Issue 3
Start page 653
End page 661
Total pages 9
Place of publication New York, United States
Publisher Springer New York LLC
Language eng
Formatted abstract
A simple microfluidic immunoassay card was developed based on polystyrene (PS) substrate for the detection of horse IgG, an inexpensive model analyte using fluorescence microscope. The primary antibody was captured onto the PS based on covalent bonding via a self-assembled monolayer (SAM) of thiol to pattern the surface chemistry on a gold-coated PS. The immunosensor chip layers were fabricated from sheets by CO2 laser ablation. The functionalized PS surfaces after each step were characterized by contact angle measurement, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). After the antibody-antigen interaction as a sandwich immunoassay with a fluorescein isothiocyanate (FITC)-conjugated secondary antibody, the intensity of fluorescence was measured on-chip to determine the concentration of the target analyte. The present immunosensor chip showed a linear response range for horse IgG between 1 μg/ml and 80 μg/ml (r = 0.971, n = 3). The detection limit was found to be 0.71 μg/ml. The developed microfluidic system can be extended for various applications including medical diagnostics, microarray detection and observing protein-protein interactions. © Springer Science+Business Media, LLC 2008.
Keyword Polystyrene
Covalent bonding
Microfluidic card
Fluorescence
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Mon, 07 Feb 2011, 16:10:45 EST by Dr Farzana Darain on behalf of Aust Institute for Bioengineering & Nanotechnology