On-chip detection of myoglobin based on fluorescence

Darain, Farzana, Yager, Paul, Gan, Kai Ling and Tjin, Swee Chuan (2009) On-chip detection of myoglobin based on fluorescence. Biosensors and Bioelectronics, 24 6: 1744-1755. doi:10.1016/j.bios.2008.09.004

Author Darain, Farzana
Yager, Paul
Gan, Kai Ling
Tjin, Swee Chuan
Title On-chip detection of myoglobin based on fluorescence
Journal name Biosensors and Bioelectronics   Check publisher's open access policy
ISSN 0956-5663
Publication date 2009-02-15
Sub-type Article (original research)
DOI 10.1016/j.bios.2008.09.004
Volume 24
Issue 6
Start page 1744
End page 1755
Total pages 12
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract
A disposable immunosensor cartridge was developed that allows antibodies to be immobilized on the surface for the detection of myoglobin, a marker for the early assessment of acute myocardial infarction (AMI) using fluorescence techniques. The anti-myoglobin antibody was immobilized on a polystyrene substrate based on covalent bonding via silanization. The immunosensor chip layers were fabricated from sheets by CO2-laser ablation. The functionalized polystyrene surfaces were characterized by contact angle measurement, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). After the antigen-antibody reaction as a sandwich enzyme-linked immunosorbent assay (ELISA) with a horseradish peroxidase-conjugated secondary antibody (HRP-anti-myoglobin), addition of fluorogenic substrate produced a fluorescent dye which was quantified on-chip using fluorescent technique. The immunosensor response was linear for myoglobin concentrations between 20 and 230 ng/ml (r = 0.991, n = 3). The detection limit was found to be 16 ng/ml, which is lower than the clinical cut-off value for myoglobin in healthy patients. This protocol could be extended to the detection of other important cardiac markers simultaneously in microchannels.
Keyword Cardiac marker
Microfluidic card
Surface modification
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Mon, 07 Feb 2011, 15:49:04 EST by Dr Farzana Darain on behalf of Aust Institute for Bioengineering & Nanotechnology