Cereal variety identification using MALDI-TOF mass spectrometry SNP genotyping

Pattemore, J. A., Rice, N., Marshall, D. F., Waugh, R. and Henry, R. J. (2010) Cereal variety identification using MALDI-TOF mass spectrometry SNP genotyping. Journal of Cereal Science, 52 3: 356-361. doi:10.1016/j.jcs.2010.06.004

Author Pattemore, J. A.
Rice, N.
Marshall, D. F.
Waugh, R.
Henry, R. J.
Title Cereal variety identification using MALDI-TOF mass spectrometry SNP genotyping
Journal name Journal of Cereal Science   Check publisher's open access policy
ISSN 0733-5210
Publication date 2010-11
Sub-type Article (original research)
DOI 10.1016/j.jcs.2010.06.004
Volume 52
Issue 3
Start page 356
End page 361
Total pages 6
Place of publication London, U.K.
Publisher Academic Press
Collection year 2011
Language eng
Formatted abstract
Correct identification of cereal varieties is important to food quality, safety and authenticity and recently, molecular markers have been applied to cereal varietal identification. Two major factors restricting the wider application of molecular markers in crop plant genetics have been throughput and cost per data point. However, in recent years, novel molecular tools, the re-invention/re-application of mature technologies, and miniaturisation of technology has both increased throughput and significantly reduced these costs. Here we have applied Sequenom® MassARRAY® MALDI-TOF mass spectrometry using a collection of recently developed SNPs to facilitate cereal varietal identification. We used a multiplexed Sequenom® MassARRAY® mass spectrometry SNP assay targeting 45 loci to genotype a collection of Australian barley varieties. Of the 45 loci screened, 33 were informative and were used to generate a unique barcode of SNPs for each variety tested. Only one variety could not be distinguished from two others due to a high level of varietal heterogeneity. This assay format provided a flexible, cost-effective, robust and moderate throughput SNP genotyping method well suited to varietal identification and purity analysis in cereals.
© 2010 Elsevier Ltd. All rights reserved.
Keyword Cereal
Variety identification
Single nucleotide polymorphism
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 06 Feb 2011, 00:07:15 EST