A defined medium and substrate for expansion of human mesenchymal stromal cell progenitors that enriches for osteo- and chondrogenic precursors

Hudson, JE, Mills, RJ, Frith, JE, Brooke, G, Jaramillo-Ferrada, P, Wolvetang, EJ and Cooper-White, JJ (2011) A defined medium and substrate for expansion of human mesenchymal stromal cell progenitors that enriches for osteo- and chondrogenic precursors. Stem Cells and Development, 20 1: 77-87.


Author Hudson, JE
Mills, RJ
Frith, JE
Brooke, G
Jaramillo-Ferrada, P
Wolvetang, EJ
Cooper-White, JJ
Title A defined medium and substrate for expansion of human mesenchymal stromal cell progenitors that enriches for osteo- and chondrogenic precursors
Journal name Stem Cells and Development  (ERA 2012 Listed)    (ERA 2010 Rank C)   Check publisher's open access policy
Publication date 2011-01-03
Sub-type Article
Year available 2010
DOI 10.1089/scd.2009.0497
Volume number 20
Issue number 1
ISSN 1547-3287; 1557-8534
Start page 77
End page 87
Total pages 11
Place of publication New Rochelle, NY, United States
Publisher Mary Ann Liebert
Collection year 2011
Language eng
Formatted abstract Human mesenchymal stromal cells (hMSCs) have generated significant interest due to their potential use in clinical applications. hMSCs are present at low frequency in vivo, but after isolation can be expanded considerably, generating clinically useful numbers of cells. In this study, we demonstrate the use of a defined embryonic stem cell expansion medium, mTeSR (Stem Cell Technologies), for the expansion of bone-marrow-derived hMSCs. The hMSCs grow at comparable rates, demonstrate tri-lineage differentiation potential, and show similar surface marker profiles (CD29+, CD44+, CD49a+, CD73+, CD90+, CD105+, CD146+, CD166+, CD34-, and CD45-) in both the fetal bovine serum (FBS)-supplemented medium and mTeSR. However, expression of early differentiation transcription factors runt-related transcription factor 2, sex-determining region Y box 9, and peroxisome proliferator-activated receptor gamma changed significantly. Both runt-related transcription factor 2 and sex-determining region Y box 9 were upregulated, whereas peroxisome proliferator-activated receptor gamma was downregulated in mTeSR compared with FBS. Although osteogenic and chondrogenic differentiation was comparable in cells grown in mTeSR compared to FBS, adipogenic differentiation was significantly decreased in mTeSR-expanded cells, both in terms of gene expression and absolute numbers of adipocytes. The removal of the FBS from the medium and the provision of a defined medium with disclosed composition make mTeSR a superior study platform for hMSC biology in a controlled environment. Further, this provides a key step toward generating a clinical-grade medium for expansion of hMSCs for clinical applications that rely on osteo- and chondroinduction of MSCs, such as bone repair and cartilage generation.
© 2011 Mary Ann Liebert, Inc.
Keyword Human bone-marrow
Embryonic stem-cells
Fetal calf serum
Human platelet lysate
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Online Ahead of Print: November 9, 2010.

 
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