Inoculation- and nitrate-induced CLE peptides of soybean control NARK-dependent nodule formation

Reid, Dugald, Ferguson, Brett J. and Gresshoff, Peter (2011) Inoculation- and nitrate-induced CLE peptides of soybean control NARK-dependent nodule formation. Molecular Plant-Microbe Interactions, 24 5: 606-618. doi:10.1094/MPMI-09-10-0207


Author Reid, Dugald
Ferguson, Brett J.
Gresshoff, Peter
Title Inoculation- and nitrate-induced CLE peptides of soybean control NARK-dependent nodule formation
Journal name Molecular Plant-Microbe Interactions   Check publisher's open access policy
ISSN 0894-0282
1943-7706
Publication date 2011-05
Sub-type Article (original research)
DOI 10.1094/MPMI-09-10-0207
Volume 24
Issue 5
Start page 606
End page 618
Total pages 13
Editor Gary Stacey
Place of publication St. Paul, MN, U.S.A.
Publisher American Phytopathological Society
Collection year 2012
Language eng
Formatted abstract
Systemic autoregulation of nodulation in legumes involves a root-derived signal (Q) that is perceived by a CLAVATA1-like leucine-rich repeat receptor kinase (e.g. GmNARK). Perception of Q triggers the production of a shoot-derived inhibitor that prevents further nodule development. We have identified three candidate CLE peptide-encoding genes (GmRIC1, GmRIC2, and GmNIC1) in soybean (Glycine max) that respond to Bradyrhizobium japonicum inoculation or nitrate treatment. Ectopic overexpression of all three CLE peptide genes in transgenic roots inhibited nodulation in a GmNARK-dependent manner. The peptides share a high degree of amino acid similarity in a 12-amino-acid C-terminal domain, deemed to represent the functional ligand of GmNARK. GmRIC1 was expressed early (12 h) in response to Bradyrhizobium-sp.-produced nodulation factor while GmRIC2 was induced later (48 to 72 h) but was more persistent during later nodule development. Neither GmRIC1 nor GmRIC2 were induced by nitrate. In contrast, GmNIC1 was strongly induced by nitrate (2 mM) treatment but not by Bradyrhizobium sp. inoculation and, unlike the other two GmCLE peptides, functioned locally to inhibit nodulation. Grafting demonstrated a requirement for root GmNARK activity for nitrate regulation of nodulation whereas Bradyrhizobium sp.-induced regulation was contingent on GmNARK function in the shoot.
Keyword Autoregulation of nodulation
CLAVATA
Legume
Long distance signalling
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
Official 2012 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 92 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 25 Jan 2011, 13:54:24 EST by Dr Brett Ferguson on behalf of Centre for Integrative Legume Research