Transcription profiling of the isoflavone phenylpropanoid pathway in soybean in response to Bradyrhizobium japonicum inoculation

Pregelj, Lisette, McLanders, Joanne R., Gresshoff, Peter M. and Schenk, Peer M. (2011) Transcription profiling of the isoflavone phenylpropanoid pathway in soybean in response to Bradyrhizobium japonicum inoculation. Functional Plant Biology, 38 1: 13-24. doi:10.1071/FP10093


Author Pregelj, Lisette
McLanders, Joanne R.
Gresshoff, Peter M.
Schenk, Peer M.
Title Transcription profiling of the isoflavone phenylpropanoid pathway in soybean in response to Bradyrhizobium japonicum inoculation
Journal name Functional Plant Biology   Check publisher's open access policy
ISSN 1445-4408
Publication date 2011
Year available 2010
Sub-type Article (original research)
DOI 10.1071/FP10093
Volume 38
Issue 1
Start page 13
End page 24
Total pages 12
Place of publication Australia
Publisher CSIRO Publishing
Collection year 2012
Language eng
Abstract Isoflavones are legume-specific secondary metabolites that function as defence compounds, signal molecules and regulators of gene expression during both pathogen attack and beneficial plant–microbe interactions. They are synthesised by a branch of the core phenylpropanoid pathway, using several isoenzymes within each enzymatic step. Gene-specific quantitative real-time reverse transcriptase PCR (qRT-PCR) was used to quantify expression of isoflavone synthesis genes in soybean (Glycine max L). Genes encoding chalcone synthase 7 (CHS7), chalcone synthase 8 (CHS8) and isoflavone synthase 1 (IFS1) displayed high basal expression levels in roots compared with hypocotyls, suggesting they could be the gene family members encoding the isoenzyme that contributes the most to the principal substrate flux towards specific isoflavone synthesis in roots. The genes encoding phenylalanine ammonia lyase 1 (PAL1) and IFS1 showed induction in root tissue after inoculation with Bradyrhizobium japonicum (Kirchner) Jordan, suggesting a control point. The absence of a functional nodulation regulator, GmNARK (G. max nodulation autoregulation receptor kinase), in the soybean mutant nts1007 resulted in significantly increased basal expression of PAL1 compared with levels induced by B. japonicum, suggesting that GmNARK is a negative regulator for isoflavone phenylpropanoid pathway genes during nodulation and that distinct genes, as opposed to the complete pathway, are coordinately regulated by the nodulation status of the mutant.
Keyword Defence signalling
Gene expression
Glycine max
GmNARK
Nitrogen fixation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Sun, 23 Jan 2011, 00:05:33 EST