Sympathetic nerve-dependent regulation of mucosal vascular tone modifies airway smooth muscle reactivity

Mazzone, SB, Lim, LHK, Wagner, EM, Mori, N and Canning, BJ (2010) Sympathetic nerve-dependent regulation of mucosal vascular tone modifies airway smooth muscle reactivity. Journal of Applied Physiology, 109 5: 1292-1300. doi:10.1152/japplphysiol.00632.2010

Author Mazzone, SB
Lim, LHK
Wagner, EM
Mori, N
Canning, BJ
Title Sympathetic nerve-dependent regulation of mucosal vascular tone modifies airway smooth muscle reactivity
Journal name Journal of Applied Physiology   Check publisher's open access policy
ISSN 8750-7587
Publication date 2010-11
Sub-type Article (original research)
DOI 10.1152/japplphysiol.00632.2010
Volume 109
Issue 5
Start page 1292
End page 1300
Total pages 9
Place of publication Bethesda, MD, United States
Publisher American Physiological Society
Collection year 2011
Language eng
Formatted abstract
The airways contain a dense subepithelial microvascular plexus that is involved in the supply and clearance of substances to and from the airway wall. We set out to test the hypothesis that airway smooth muscle reactivity to bronchoconstricting agents may be dependent on airway mucosal blood flow. Immunohistochemical staining identified vasoconstrictor and vasodilator nerve fibers associated with subepithelial blood vessels in the guinea pig airways. Intravital microscopy of the tracheal mucosal microvasculature in anesthetized guinea pigs revealed that blockade of α-adrenergic receptors increased baseline arteriole diameter by ∼40%, whereas the α-adrenergic receptor agonist phenylephrine produced a modest (5%) vasoconstriction in excess of the baseline tone. In subsequent in vivo experiments, tracheal contractions evoked by topically applied histamine were significantly reduced (P <0.05) and enhanced by α-adrenergic receptor blockade and activation, respectively. α-Adrenergic ligands produced similar significant (P <0.05) effects on airway smooth muscle contractions evoked by topically administered capsaicin, intravenously administered neurokinin A, inhaled histamine, and topically administered antigen in sensitized animals. These responses were independent of any direct effect of α-adrenergic ligands on the airway smooth muscle tone. The data suggest that changes in blood flow in the vessels supplying the airways regulate the reactivity of the underlying airway smooth muscle to locally released and exogenously administered agents by regulating their clearance. We speculate that changes in mucosal vascular function or changes in neuronal regulation of the airway vasculature may contribute to airways responsiveness in disease. © 2010 the American Physiological Society.
Keyword Tracheobronchial vasculature
Intravital microscopy
Particle clearance
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biomedical Sciences Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 14 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 16 Jan 2011, 00:06:27 EST