Effect of chemical and physical treatments on the in vitro germination and growth of 'Laguna Tall' coconut embryos

Magdalita, PM, Damasco, OP and Adkins, SW (2010) Effect of chemical and physical treatments on the in vitro germination and growth of 'Laguna Tall' coconut embryos. Philippine Journal of Crop Science, 35 3: 41-49.

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Name Description MIMEType Size Downloads
Author Magdalita, PM
Damasco, OP
Adkins, SW
Title Effect of chemical and physical treatments on the in vitro germination and growth of 'Laguna Tall' coconut embryos
Formatted title
Effect of chemical and physical treatments on the in vitro germination and growth of 'Laguna Tall' coconut embryos
Journal name Philippine Journal of Crop Science
ISSN 0115-2025
0115-463X
Publication date 2010-12
Sub-type Article (original research)
Open Access Status
Volume 35
Issue 3
Start page 41
End page 49
Total pages 9
Place of publication Los Banos, Laguna, Philippines
Publisher University of the Philippines Los Banos, Crop Science Society of the Philippines
Collection year 2011
Language eng
Formatted abstract
In vitro studies involving 'Laguna Tall' coconut embryos were conducted to assess the germination and growth of seedlings in response to different chemicals added to the Y3 medium, manner of culture and different light spectra treatments during incubation. Supplementation of gibberellic acid at 30 and 40 μM into the Y3 medium significantly promoted embryo germination and shoot emergence of seedlings, but at a higher concentration of 50 μM, germination was significantly inhibited. Similarly, supplementation of 10-40 μM GA(3) promoted shoot elongation of germinated coconut seedlings, but 50 μM GA(3) inhibited shoot elongation. Abscisic acid at 0.94-3.78 μ M added to the Y3 medium also promoted germination of embryos, but higher concentration of 7.67 μM significantly decreased germination. However, silver thiosulfate, spermidine and the state of culture had no effect on embryo germination and growth of seedlings. The different visible light spectra i.e. blue (430-500 nm), green (500-560 nm), yellow (560-600 nm) and red (650-750 nm) significantly affected fresh weight and shoot length of germinated seedlings 16 weeks after explanting. Incubation of cultures under red light spectrum (650-750 nm) significantly increased the fresh weight of coconut seedlings compared to those seedlings incubated under white fluorescent light.
Keyword Abscisic acid
Coconut embryo
Germination
Gibberellic acid
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
 
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Created: Sun, 16 Jan 2011, 00:02:12 EST