Dehydration improves cryopreservation of coconut (Cocos nucifera L.)

Sisunandar, Sopade, Peter A., Samosir Yohannes M. S., Rival, Alain and Adkins, Steve W. (2010) Dehydration improves cryopreservation of coconut (Cocos nucifera L.). Cryobiology, 61 3: 289-296. doi:10.1016/j.cryobiol.2010.09.007


Author Sisunandar
Sopade, Peter A.
Samosir Yohannes M. S.
Rival, Alain
Adkins, Steve W.
Title Dehydration improves cryopreservation of coconut (Cocos nucifera L.)
Formatted title
Dehydration improves cryopreservation of coconut (Cocos nucifera L.)
Journal name Cryobiology   Check publisher's open access policy
ISSN 0011-2240
1090-2392
Publication date 2010-12
Sub-type Article (original research)
DOI 10.1016/j.cryobiol.2010.09.007
Volume 61
Issue 3
Start page 289
End page 296
Total pages 8
Place of publication Maryland Heights, United States
Publisher Academic Press
Collection year 2011
Language eng
Formatted abstract
Cryopreservation of coconut can be used as a strategy to back up the establishment of living collections
which are expensive to maintain and are under constant threat from biotic and abiotic factors. Unfortunately,
cryopreservation protocols still need to be developed that are capable of producing a sizeable number
of field-grown plants. Therefore, we report on the development of an improved cryopreservation
protocol which can be used on a wide range of coconut cultivars. The cryopreservation of zygotic embryos
and their recovery to soil-growing plants was achieved through the application of four optimised steps viz.:
(i) rapid dehydration; (ii) rapid cooling; (iii) rapid warming and recovery in vitro and (iv) acclimatisation
and soil-supported growth. The thermal properties of water within the embryos were monitored using differential
scanning calorimetry (DSC) in order to ensure that the freezable component was kept to a minimum.
The feasibility of the protocol was assessed using the Malayan Yellow Dwarf (MYD) cultivar in
Australia and then tested on a range of cultivars which were freshly harvested and studied in Indonesia.
The most efficient protocol was one based on an 8-h rapid dehydration step followed by rapid cooling step.
Best recovery percentages were obtained when a rapid warming step and an optimised in vitro culture step
were used. Following this protocol, 20% (when cryopreserved 12 days after harvesting) and 40% (when
cryopreserved at the time of harvest) of all MYD embryos cryopreserved could be returned to normal seedlings
growing in soil. DSC showed that this protocol induced a drop in embryo fresh weight to 19% and significantly
reduced the amount of water remaining that could produce ice crystals (0.1%). Of the 20 cultivars
tested, 16 were found to produce between 10% and 40% normal seedlings while four cultivars generated
between 0% and 10% normal seedlings after cryopreservation. This new protocol is applicable to a wide
range of coconut cultivars and is useful for the routine cryopreservation of coconut genetic resources. © 2010 Elsevier Inc. All rights reserved.
Keyword Dehydration
Differential scanning calorimetry
Germplasm conservation
Embryo culture
Recalcitrant seeds
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Official 2011 Collection
 
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Created: Sun, 09 Jan 2011, 00:09:39 EST