Granule formation mechanisms within an aerobic wastewater system for phosphorus removal

Barr, Jeremy J., Cook, Andrew E. and Bond, Phillip L. (2010) Granule formation mechanisms within an aerobic wastewater system for phosphorus removal. Applied and Environmental Microbiology, 76 22: 7588-7597. doi:10.1128/AEM.00864-10

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Author Barr, Jeremy J.
Cook, Andrew E.
Bond, Phillip L.
Title Granule formation mechanisms within an aerobic wastewater system for phosphorus removal
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
Publication date 2010-11
Sub-type Article (original research)
DOI 10.1128/AEM.00864-10
Open Access Status File (Publisher version)
Volume 76
Issue 22
Start page 7588
End page 7597
Total pages 10
Editor L. Nicholas Ornston
Place of publication Washington, DC, U.S.A.
Publisher American Society for Microbiology
Collection year 2011
Language eng
Formatted abstract
Granular sludge is a novel alternative for the treatment of wastewater and offers numerous operational and economic advantages over conventional floccular-sludge systems. The majority of research on granular sludge has focused on optimization of engineering aspects relating to reactor operation with little emphasis on the fundamental microbiology. In this study, we hypothesize two novel mechanisms for granule formation as observed in three laboratory scale sequencing batch reactors operating for biological phosphorus removal and treating two different types of wastewater. During the initial stages of granulation, two distinct granule types (white and yellow) were distinguished within the mixed microbial population. White granules appeared as compact, smooth, dense aggregates dominated by 97.5% "Candidatus Accumulibacter phosphatis," and yellow granules appeared as loose, rough, irregular aggregates with a mixed microbial population of 12.3% "Candidatus Accumulibacter phosphatis" and 57.9% "Candidatus Competibacter phosphatis," among other bacteria. Microscopy showed white granules as homogeneous microbial aggregates and yellow granules as segregated, microcolony-like aggregates, with phylogenetic analysis suggesting that the granule types are likely not a result of strain-associated differences. The microbial community composition and arrangement suggest different formation mechanisms occur for each granule type. White granules are hypothesized to form by outgrowth from a single microcolony into a granule dominated by one bacterial type, while yellow granules are hypothesized to form via multiple microcolony aggregation into a microcolony-segregated granule with a mixed microbial population. Further understanding and application of these mechanisms and the associated microbial ecology may provide conceptual information benefiting start-up procedures for full-scale granular-sludge reactors.
Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Keyword Sequencing batch reactor
16S ribosomal-RNA
Glycogen-accumulating organisms
Targeted oligonucleotide probes
Polyphosphate kinase genes
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Supplemental material (read only) -

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Advanced Water Management Centre Publications
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Citation counts: TR Web of Science Citation Count  Cited 17 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 18 times in Scopus Article | Citations
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Created: Fri, 07 Jan 2011, 14:12:36 EST by Hong Lee on behalf of Advanced Water Management Centre