An improved extraction method for the HPLC determination of morphine and its metabolites in plasma

Pawula, Maria, Barrett, David A. and Shaw, P. Nicholas (1993) An improved extraction method for the HPLC determination of morphine and its metabolites in plasma. Journal of Pharmaceutical and Biomedical Analysis, 11 4-5: 401-406. doi:10.1016/0731-7085(93)80036-Z


Author Pawula, Maria
Barrett, David A.
Shaw, P. Nicholas
Title An improved extraction method for the HPLC determination of morphine and its metabolites in plasma
Journal name Journal of Pharmaceutical and Biomedical Analysis   Check publisher's open access policy
ISSN 0731-7085
1873-264X
Publication date 1993-04
Sub-type Article (original research)
DOI 10.1016/0731-7085(93)80036-Z
Volume 11
Issue 4-5
Start page 401
End page 406
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
A new, simple and rapid extraction procedure coupled with a combined coulometric-fluorescence HPLC assay is described for the simultaneous determination of morphine (M) and morphine-3-glucuronide (M3G), morphine-6-glucuronide (M6G), and normorphine (NM) in plasma. The effect of concentration and pH of selected ion-pairing agents on the extraction of these compounds from plasma by solid-phase extraction was investigated. The extraction procedure was optimized in terms of recovery, reproducibility and lack of interference from endogenous materials. The optimized method uses tetrabutylammonium hydrogen sulphate (TBAHS) at pH 10 followed by separation on a single C18 solid-phase extraction cartridge. For routine analysis the procedure provides high and reproducible recoveries over a concentation range of 1.0-1000 ng ml-1 for morphine, M6G and normorphine and 20-1000 ng ml-1 for M3G. The method was used successfully to analyse plasma samples from a pharmacokinetic study in which sheep had received an intravenous dose of 0.015 mg kg-1 of M6G.
Keyword High-performance liquid chromatography
Morphine
Morphine-6-glucuronide
Morphine-3-glucuronide
Solid-phase extraction
Plasma
Performance liquid-chromatography
Electrochemical detection
Biological samples
Urine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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Created: Mon, 20 Dec 2010, 14:09:39 EST