Rapid and selective high-performance liquid chromatographic method for the determination of metronidazole and its active metabolite in human plasma, saliva and gastric juice

Jessa, M.J., Barrett, D.A., Shaw, P.N. and Spiller, R.C. (1996) Rapid and selective high-performance liquid chromatographic method for the determination of metronidazole and its active metabolite in human plasma, saliva and gastric juice. Journal of Chromatography. B, Biomedical Applications, 677 2: 374-379. doi:10.1016/0378-4347(95)00421-1


Author Jessa, M.J.
Barrett, D.A.
Shaw, P.N.
Spiller, R.C.
Title Rapid and selective high-performance liquid chromatographic method for the determination of metronidazole and its active metabolite in human plasma, saliva and gastric juice
Journal name Journal of Chromatography. B, Biomedical Applications
ISSN 0378-4347
Publication date 1996-03
Sub-type Article (original research)
DOI 10.1016/0378-4347(95)00421-1
Volume 677
Issue 2
Start page 374
End page 379
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract A rapid and selective HPLC method has been developed for the separation and quantitation of metronidazole and its hydroxylated metabolite in human plasma, saliva and gastric juice. The assay requires a simple protein precipitation step prior to analysis and is selective, sensitive and reproducible. The limits of quantitation (0/5-ml sample) were at least 0.25 μg/ml for metronidazole and 0.20 μg/ml for its hydroxy metabolite. A Hypersil ODS 5 μm (150×4.6 mm I.D.) column was used with a mobile phase of acetonitrile-aqueous 0.05 M potassium phosphate buffer (pH 7) containing 0.1% triethylamine (10:90) delivered at a flow-rate of 1.0 ml/min.
Keyword Metronidazole
Hydroxymetronidazole
Human-serum
Pharmacokinetics
Bioavailability
Tinidazole
Assay
Urine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Short communication

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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