The analysis of pharmaceutical bases on a silica stationary phase by capillary electrochromatography using aqueous mobile phases

Gillott, N.C., Euerby, M.R., Johnson, C.M., Barrett, D.A. and Shaw, P.N. (2000) The analysis of pharmaceutical bases on a silica stationary phase by capillary electrochromatography using aqueous mobile phases. Chromatographia, 51 3-4: 167-174. doi:10.1007/BF02490560


Author Gillott, N.C.
Euerby, M.R.
Johnson, C.M.
Barrett, D.A.
Shaw, P.N.
Title The analysis of pharmaceutical bases on a silica stationary phase by capillary electrochromatography using aqueous mobile phases
Journal name Chromatographia   Check publisher's open access policy
ISSN 0009-5893
1612-1112
Publication date 2000-02
Sub-type Article (original research)
DOI 10.1007/BF02490560
Volume 51
Issue 3-4
Start page 167
End page 174
Total pages 8
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
The capillary electrochromatographic (CEC) separation of a range of pharmaceutical bases was investigated on a commercially available silica stationary phase using aqueous mobile phases. The effects of mobile phase composition, buffer pH, applied voltage, and buffer anion on the retention behaviour of these bases were studied. Promising chromatography was obtained at pH 7.8 but was later found to be irreproducible. However, successful and reproducible chromatography of the bases was achieved at pH 2.3.
We have previously demonstrated that the addition of mobile phase additives such as TEA-phosphate at low pH values has resulted in excellent CEC analysis of bases on reversed-phase packing materials. The same approach was applied to the analysis of bases on the silica phase in order to improve peak shape. Excellent chromatography was obtained for the analysis of strong pharmaceutical bases such as benzylamine, nortriptyline and diphenhydramine.
The experimental investigations have shown that the CEC separation of a range of pharmaceutical bases can routinely be achieved with excellent peak shapes and peak efficiencies as high as 320,000 plates m−1.
Keyword Capillary electrochromatography
Pharmaceutical bases
Silica stationary phases
Aqueous mobile phases
Bare silica
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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Created: Mon, 20 Dec 2010, 14:07:14 EST