Separation of pharmaceutical bases from neutral and acidic components by capillary electrochromatography

Gillott, Nicola C., Euerby, Melvin R., Johnson, Christopher M., Barrett, David A. and Shaw, P. Nicholas (1998) Separation of pharmaceutical bases from neutral and acidic components by capillary electrochromatography. Analytical Communications, 35 7: 217-220. doi:10.1039/a803876g


Author Gillott, Nicola C.
Euerby, Melvin R.
Johnson, Christopher M.
Barrett, David A.
Shaw, P. Nicholas
Title Separation of pharmaceutical bases from neutral and acidic components by capillary electrochromatography
Journal name Analytical Communications   Check publisher's open access policy
ISSN 1359-7337
1364-5536
Publication date 1998-07
Sub-type Article (original research)
DOI 10.1039/a803876g
Volume 35
Issue 7
Start page 217
End page 220
Total pages 4
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Language eng
Formatted abstract
Capillary electrochromatography (CEC) has proved to be applicable to the separation of neutral and acidic (chromatographed in their ion-suppressed mode) compounds but a major factor holding back its further development has been its perceived inability to analyse basic compounds. In this paper we demonstrate that the addition of a competing base, such as triethylamine (TEA) or triethanolamine (TEOA), to a low pH buffer can achieve excellent CEC analysis of a range of strong pharmaceutical bases. Acceptable peak symmetry and efficiencies of up to 510000 plates m–1 were obtained for bases such as procainamide, nortriptyline and diphenhydramine on CEC Hypersil C18, C8 and phenyl reversed-phase packing materials. The effect of TEA or TEOA concentration and mobile phase pH on the separation behaviour of the bases is discussed. In addition, the paper reports the first example of the simultaneous CEC analysis of acids, bases and neutral compounds by using a mobile phase containing TEOA phosphate at pH 2.5.
Keyword Reversed-phase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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Created: Mon, 20 Dec 2010, 14:07:08 EST