The C-S lysis of L-cysteine conjugates by aspartate and alanine aminotransferase enzymes

Gaskin, Peter J., Adcock, Harriet J., Buckberry, Lorraine D., Teesdale-Spittle, Paul H and Shaw, P. Nicholas (1995) The C-S lysis of L-cysteine conjugates by aspartate and alanine aminotransferase enzymes. Human and Experimental Toxicology, 14 5: 422-427.


Author Gaskin, Peter J.
Adcock, Harriet J.
Buckberry, Lorraine D.
Teesdale-Spittle, Paul H
Shaw, P. Nicholas
Title The C-S lysis of L-cysteine conjugates by aspartate and alanine aminotransferase enzymes
Journal name Human and Experimental Toxicology   Check publisher's open access policy
ISSN 0144-5952
1477-0903
Publication date 1995-05
Sub-type Article (original research)
Volume 14
Issue 5
Start page 422
End page 427
Total pages 6
Place of publication London, United Kingdom
Publisher Sage Publications
Language eng
Abstract One biotransformation pathway which is responsible for the generation of mutagenic and cytotoxic metabolites is that of the C-S lysis (CSL) of L-cysteine conjugates. Thirteen cysteine S-conjugates, synthesised in our laboratories, were incubated with porcine heart aspartate aminotransferase (ASAT) and alanine aminotransferase (ALAT), and the C-S lyase activity for each enzyme-substrate combination was determined. ASAT and ALAT were shown to exhibit CSL activity. It was also demonstrated that this activity was inhibited in the presence of the pyridoxal phosphate [PLP]-dependent enzyme inhibitor amino(oxyacetic acid) (AOAA) confirming the pyridoxal phosphate dependent mechanism by which C-S lysis is known to take place. Since the activities of these enzymes are used as biomarkers for the assessment of organ damage, the potential interaction of L-cysteine conjugates with them may suppress their activity through direct inhibition.
Keyword Cysteine conjugate beta-lyase
Aspartate amino transferase
Alanine amino transferase
Porcine heart
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Continues Human toxicology

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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Created: Mon, 20 Dec 2010, 14:07:01 EST