Solvent and stationary phase selectivity in capillary electrochromatography method development: Comparison of C18, C8, and phenyl-bonded phases for the separation of a series of substituted barbiturates

Euerby, M. R., Johnson, C. M., Smyth, S. F., Gillott, N., Barrett, D. A. and Shaw, P. N. (1999). Solvent and stationary phase selectivity in capillary electrochromatography method development: Comparison of C18, C8, and phenyl-bonded phases for the separation of a series of substituted barbiturates. In: 20th International Symposium on Capillary Chromatography and Electrophoresis, Riva del Garda, Italy, (305-311). May 1998. doi:10.1002/(SICI)1520-667X(1999)11:4<305::AID-MCS8>3.0.CO;2-G


Author Euerby, M. R.
Johnson, C. M.
Smyth, S. F.
Gillott, N.
Barrett, D. A.
Shaw, P. N.
Title of paper Solvent and stationary phase selectivity in capillary electrochromatography method development: Comparison of C18, C8, and phenyl-bonded phases for the separation of a series of substituted barbiturates
Conference name 20th International Symposium on Capillary Chromatography and Electrophoresis
Conference location Riva del Garda, Italy
Conference dates May 1998
Journal name Journal of Microcolumn Separations   Check publisher's open access policy
Place of Publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Publication Year 1999
Sub-type Fully published paper
DOI 10.1002/(SICI)1520-667X(1999)11:4<305::AID-MCS8>3.0.CO;2-G
ISSN 1040-7685
1520-667X
Volume 11
Issue 4
Start page 305
End page 311
Total pages 6
Language eng
Abstract/Summary The capillary electrochromatographic separation of six substituted barbiturates in the ion-suppressed mode was investigated on commercially available C18, C8, and phenyl-bonded packing materials. The effect on the retention behavior of mobile phase composition, buffer pH, buffer ionic strength, and temperature was studied. Successful chromatography of the barbiturates was achieved with all three packing materials over a pH range from 2.3 to 7.8. It was notable that a significant electroosmotic flow (EOF) was generated with a pH 2.3 mobile phase for all the packing materials, suggesting that capillary electrochromatography (CEC) in the ion suppression mode can be applied to a wide range of weakly acidic compounds of pharmaceutical interest. The C18, C8, and phenyl-bonded packing materials gave similar selectivities and retention times, and approximately linear relationships between percentage organic modifier in the mobile phase or temperature versus ln k were observed. Increasing the buffer ionic strength reduced the EOF but did not result in changes in selectivity. The use of methanol compared with acetonitrile had no effect on selectivity but resulted in a large increase in retention of the analytes. The experimental investigations have shown that the CEC separation of a series of weakly acidic compounds of pharmaceutical relevance can be achieved readily using a range of commercially available packed capillaries.
Keyword Capillary Electrochromatography
Mobile And Stationary Phase Selectivity
Barbiturates
Pharmaceutical Applications
Mobile-phase
Behavior
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Conference Paper
Collection: School of Pharmacy Publications
 
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Created: Mon, 20 Dec 2010, 14:06:37 EST