Reversed-phase high-performance liquid chromatographic assay methods for the analysis of a range of penicillins in in vitro permeation studies

Erah, P.O., Barrett, D.A. and SHAW, P.N. (1998) Reversed-phase high-performance liquid chromatographic assay methods for the analysis of a range of penicillins in in vitro permeation studies. Journal of Chromatography B: Biomedical Applications, 705 1: 63-69. doi:10.1016/S0378-4347(97)00484-2


Author Erah, P.O.
Barrett, D.A.
SHAW, P.N.
Title Reversed-phase high-performance liquid chromatographic assay methods for the analysis of a range of penicillins in in vitro permeation studies
Journal name Journal of Chromatography B: Biomedical Applications
ISSN 0378-4347
Publication date 1998-01
Sub-type Article (original research)
DOI 10.1016/S0378-4347(97)00484-2
Volume 705
Issue 1
Start page 63
End page 69
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Simple, rapid and selective HPLC methods have been developed for the analysis of amoxycillin, ampicillin, epicillin, mecillinam and propicillin. A Hypersil ODS 5 μm (150 x 4.6 mm I.D.) column was used with mobile phases containing aqueous phosphate buffers, pH 3-4.6 and either methanol or acetonitrile as the organic modifier. Samples were detected by their optimal UV absorption (210-230 nm). The lower limits of quantitation of the compounds (100 μl injection volume) were 0.1 μg/ml. The assays were linear in the range of 0.1-100 μg/ml with r2 values greater than 0.99. The methods have been applied successfully for the measurement of the flux of the compounds across Caco-2 cells monolayers.
Keyword Penicillins
Beta-lactam antibiotics
Physicochemical properties
Ampicillin
Dosage
Fluids
Ph
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Continued by Journal of chromatography. B, Biomedical applications and applications

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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