Cysteine conjugate toxicity in a human cell line: Correlation with C-S lyase activity in human hepatic tissue

Buckberry, Lorraine D., Blagbrough, Ian S. and Shaw, P. Nicholas (1993) Cysteine conjugate toxicity in a human cell line: Correlation with C-S lyase activity in human hepatic tissue. Human and Experimental Toxicology, 12 4: 329-335.


Author Buckberry, Lorraine D.
Blagbrough, Ian S.
Shaw, P. Nicholas
Title Cysteine conjugate toxicity in a human cell line: Correlation with C-S lyase activity in human hepatic tissue
Journal name Human and Experimental Toxicology   Check publisher's open access policy
ISSN 0144-5952
1477-0903
Publication date 1993-07-01
Sub-type Article (original research)
Volume 12
Issue 4
Start page 329
End page 335
Total pages 7
Place of publication London, United Kingdom
Publisher Sage Publications
Language eng
Abstract C-S lyase enzymes catalyse the generation of mutagenic and/or cytotoxic thiols from cysteine conjugated xenobiotics. These cysteine conjugates are produced subsequent to glutathione conjugations as a metabolic step in the mercapturic acid pathway, traditionally thought of as a pathway solely associated with detoxification. Human Chang liver (HCL) cells were challenged with a range of cysteine conjugates demonstrated to be substrates for human hepatic C-S lyases. The cellular toxicity of these compounds was determined and it was observed that the rank order of substrate toxicity obtained for the HCL cells followed the rank order of C-S lyase activity of the substrates in a freshly isolated mitochondrial fraction of human tissue. The presence of C-S lyase activity was also established in this cell line.
Keyword Beta-lyase
Rat-liver
Nephrotoxicity
Mechanism
Bioactivation
Purification
Metabolites
Enzyme
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Continues Human toxicology

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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Created: Tue, 21 Dec 2010, 00:05:11 EST