Bovine pulmonary, hepatic and renal tissues: models for the study of mammalian C-S lyase enzymes

Buckberry, L.D., Blagbrough, I.S., Bycroft, B.W. and Shaw, P.N. (1993) Bovine pulmonary, hepatic and renal tissues: models for the study of mammalian C-S lyase enzymes. Alternatives to Laboratory Animals, 21 3: 360-370.

Author Buckberry, L.D.
Blagbrough, I.S.
Bycroft, B.W.
Shaw, P.N.
Title Bovine pulmonary, hepatic and renal tissues: models for the study of mammalian C-S lyase enzymes
Journal name Alternatives to Laboratory Animals   Check publisher's open access policy
Publication date 1993-07
Sub-type Article
Volume number 21
Issue number 3
ISSN 0261-1929
Start page 360
End page 370
Total pages 11
Place of publication Nottingham, United Kingdom
Publisher Fund for the Replacement of Animals in Medical Experiments
Language eng
Abstract C-S lyase (CSL) enzymes are responsible for the generation of toxicity via the cleavage of cysteine conjugates to generate reactive thiol species, In order to explore and characterise CSL activity in mammalian organs, cysteine conjugate CSL enzymes were isolated from bovine pulmonary, hepatic and renal tissues. Bovine tissue, obtained from the abbatoir, affords a readily available source of viable CSL enzymes, without the necessity of sacrificing large numbers of laboratory animals simply to provide tissue. We have demonstrated that significant CSL activity exists in bovine tissues, and that the level of this activity is comparable with that found in human tissues. These enzymes provide an explanation for the previously reported episodes of bovine toxicity, and may provide a reasonable model for other mammalian CSL enzymes.
Keyword C-s lyase
Cysteine conjugate beta-lyase
Bovine pulmonary
Hepatic and renal tissue
S-(e-1,2-dichlorovinyl)-l-cysteine
Dcvc
Conjugate beta-lyase
Glutamine transaminase-k
Rat-liver
Cysteine
Purification
S-(1,2-dichlorovinyl)-l-cysteine
Identity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article
Collection: School of Pharmacy Publications
 
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Created: Mon, 20 Dec 2010, 14:05:04 EST