Determination of morphine and 6-acetylmorphine in plasma by high-perfromance liquid chromatography with fluorescence detection

Barrett, D.A., Shaw, P.N. and Davis, S.S. (1991) Determination of morphine and 6-acetylmorphine in plasma by high-perfromance liquid chromatography with fluorescence detection. Journal of Chromatography. Biomedical Applications, 566 1: 135-145. doi:10.1016/0378-4347(91)80118-V


Author Barrett, D.A.
Shaw, P.N.
Davis, S.S.
Title Determination of morphine and 6-acetylmorphine in plasma by high-perfromance liquid chromatography with fluorescence detection
Journal name Journal of Chromatography. Biomedical Applications
ISSN 0378-4347
Publication date 1991-05
Sub-type Article (original research)
DOI 10.1016/0378-4347(91)80118-V
Volume 566
Issue 1
Start page 135
End page 145
Total pages 11
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract A method is described for the simultaneous determination of morphine and 6-acetylmorphine is small volumes of human plasma by normal-phase high-performance liquid chromatography using solid-phase extraction, dansyl derivatisation and fluorescence detection. The lower limits of quantitation in a 0.1-ml plasma sample are 10 ng/ml for morphine and 25 ng/ml for 6-acetylmorphine. The method has been applied to determine concentrations of morphine and 6-acetylmorphine in plasma samples from premature babies administered an intravenous infusion of diamorphine.
Keyword Diacetylmorphine Heroin
Biological Samples
Pharmacokinetics
Metabolites
Separation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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Created: Mon, 20 Dec 2010, 14:04:15 EST