A stability indicating HPLC assay for diamorphine in aqueous solution

Barrett, David A. and Shaw, P.Nicholas (1994) A stability indicating HPLC assay for diamorphine in aqueous solution. Journal of Liquid Chromatography, 17 17: 3727-3733. doi:10.1080/10826079408013988


Author Barrett, David A.
Shaw, P.Nicholas
Title A stability indicating HPLC assay for diamorphine in aqueous solution
Journal name Journal of Liquid Chromatography   Check publisher's open access policy
ISSN 0148-3919
Publication date 1994-10
Sub-type Article (original research)
DOI 10.1080/10826079408013988
Volume 17
Issue 17
Start page 3727
End page 3733
Total pages 7
Place of publication Philadelphia, PA, United States
Publisher Taylor & Francis
Language eng
Formatted abstract A method is described for the rapid and simultaneous determination of diamorphine, and its hydrolysis products, 6-acetylmorphine and morphine using high-performance liquid chromatography. The column used was a Hypersil BDS C18 (100 x 4.6 mm) and the mobile phase comprised 80% aqueous potassium hydrogen phosphate (0.5 M), 20% acetonitrile, adjusted to pH 3.0 with orthophosphoric acid. The flow rate was 1.0 ml.min-1 and the limit of detection of each compound was 0.01 μg.ml-1. The assay was linear over the range of 0.01 to 100 μg.ml-1. The inter and intra-assay reproducibilities were better than 4% over this range of concentrations. The assay method was used to determine the effect of temperature on the degradation of diamorphine at pH 7.4 in aqueous solution.

A method is described for the rapid and simultaneous determination of diamorphine, and its hydrolysis products, 6-acetylmorphine and morphine using high-performance liquid chromatography. The column used was a Hypersil BDS C18 (100×4.6 mm) and the mobile phase comprised 80% aqueous potassium hydrogen phosphate (0.05M), 20% acetonitrile, adjusted to pH 3.0 with orthophosphoric acid. The flow rate was 1.0 ml.min-1 and the limit of detection of each compound was 0.01 μg.ml-1. The assay was linear over the range of 0.01 to 100 μg.ml-1. The inter and intra-assay reproducibilities were better than 4% over this range of concentrations. The assay method was used to determine the effect of temperature on the degradation of diamorphine at pH 7.4 in aqueous solution.
Keyword Deacetylation
Infusion
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Continued by Journal of Liquid Chromatography and Related Technologies

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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