Retention behavior of morphine and its metabolites on a porous graphitic carbon column

Barrett, D.A., Pawula, M., Knaggs, R.D. and Shaw, P.N. (1998) Retention behavior of morphine and its metabolites on a porous graphitic carbon column. Chromatographia, 47 11-12: 667-672. doi:10.1007/BF02467451


Author Barrett, D.A.
Pawula, M.
Knaggs, R.D.
Shaw, P.N.
Title Retention behavior of morphine and its metabolites on a porous graphitic carbon column
Journal name Chromatographia   Check publisher's open access policy
ISSN 0009-5893
1612-1112
Publication date 1998-06
Sub-type Article (original research)
DOI 10.1007/BF02467451
Volume 47
Issue 11-12
Start page 667
End page 672
Total pages 6
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract The chromatographic behaviour of a series of morphine-based opiates has been investigated using a porous graphitic carbon packing material at acid and alkaline pH. The effects of mobile phase pH, mobile phase organic percentage, column temperature and ion-pairing agents were studied. All six opiates were separated within a close retention window despite large differences in measured lipophilicities of the individual opiates. The retention order was not related to the log P values of the opiates and strong retention of the fully ionised compounds was observed, particularly those with acidic functional groups. The effect of pH on the retention of the compounds indicated that the degree of ionisation of the individual compounds was important in the separation mechanism, suggesting that hydrophobic interactions were present in addition to the polar retentive effects observed above. The strong retention of the ionised glucuronide and sulphate conjugates of morphine is a particularly useful feature of the porous graphitic carbon packing material which has general applicability to the analysis of polar or ionised drug metabolites.
Keyword Column liquid chromatography
Porous graphitic carbon
Optimization
Morphine and metabolites
Performance liquid-chromatography
Silica
Plasma
Hplc
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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Created: Mon, 20 Dec 2010, 14:04:04 EST