Characterization of a range of alkyl-bonded silica HPLC stationary phases: Chromatographic behavior of neutral, acidic, and basic test solutes

Barrett, David A., Brown, Vikki A., Shaw, P. Nicholas, Davies, Martyn C., Ritchie, Harald and Ross, Paul (1996) Characterization of a range of alkyl-bonded silica HPLC stationary phases: Chromatographic behavior of neutral, acidic, and basic test solutes. Journal of Chromatographic Science, 34 3: 146-156.


Author Barrett, David A.
Brown, Vikki A.
Shaw, P. Nicholas
Davies, Martyn C.
Ritchie, Harald
Ross, Paul
Title Characterization of a range of alkyl-bonded silica HPLC stationary phases: Chromatographic behavior of neutral, acidic, and basic test solutes
Journal name Journal of Chromatographic Science   Check publisher's open access policy
ISSN 0021-9665
1945-239X
Publication date 1996-03
Sub-type Article (original research)
Volume 34
Issue 3
Start page 146
End page 156
Total pages 11
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Language eng
Formatted abstract The effect of the chain length (C1-C18) of n-alkyl bonded-phase silica packing materials on retention behavior is investigated using three series of substituted test solutes: biphenyls (neutral), barbiturates (weakly acidic), and pyridines (weakly basic). An excellent correlation between the capacity factor (k) and alkyl chain length is observed in the majority of cases. However, deviations from this relationship occur when the alkyl chain length is increased beyond C8 for certain groups of solutes or when there is significant solute interaction at secondary sites on the packing material. Log k plots showed that the neutral and barbiturate solutes have similar retention mechanisms at all the alkyl chain lengths. However, the basic solutes show significant evidence of secondary interactions, which are confirmed by the peak asymmetry data and the lack of correlation between the log10 octanol-water partition coefficient (log P) values of the solutes and retention. There is a significant trend towards improved peak symmetry for the basic solutes when the packing materials having the highest alkyl surface density (C1, C2, and C3 alkyl chain lengths) are used; this suggests that residual silanols are more effectively masked by the shorter alkyl chain lengths. The neutral biphenyl solutes show a progressive increase in efficiency as the chain length increases, but consistent trends are not observed for the acidic and basic solutes. The results show that the length of the bonded alkyl chain is of primary, though not sole, importance in governing the retention of acidic, basic, and neutral compounds on a series of monofunctional n-alkyl-bonded reversed-phase packing materials. Retention mechanisms that might explain the observed separation behavior are discussed.
Keyword Performance liquid-chromatography
Reversed-phase
Retention mechanism
Surface silanols
Chain-length
Selectivity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 21 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 23 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Access Statistics: 31 Abstract Views  -  Detailed Statistics
Created: Mon, 20 Dec 2010, 14:03:49 EST