Measurement of dothiepin and its major metabolites in plasma by high-performance liquid chromatography

Taylor, P.J., Charles, B. G., Norris, R., Salm, P. and Ravenscroft, P. J. (1992) Measurement of dothiepin and its major metabolites in plasma by high-performance liquid chromatography. Journal of Chromatography B, 581 1: 152-155. doi:10.1016/0378-4347(92)80460-8


Author Taylor, P.J.
Charles, B. G.
Norris, R.
Salm, P.
Ravenscroft, P. J.
Title Measurement of dothiepin and its major metabolites in plasma by high-performance liquid chromatography
Journal name Journal of Chromatography B
ISSN 0378-4347
1873-376X
Publication date 1992-10
Sub-type Article (original research)
DOI 10.1016/0378-4347(92)80460-8
Volume 581
Issue 1
Start page 152
End page 155
Total pages 4
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
This paper describes a reversed-phase high-performance liquid chromatographic method which will simultaneously measure dothiepin and its three major metabolites (northiaden, northiaden-S-oxide and dothiepin-S-oxide) in plasma using trimipramine as internal standard. Sample preparation involved a basic extraction using diethyl ether followed by an acid back-extraction. The method we report is linear over the range 50–1000 ng/ml (r = 0.999), for all analytes. Total imprecision is less than 11% (coefficient of variation) and accuracy is greater than 94% (n = 20). Recovery of analytes varied considerably from 51.7% for northiaden-S-oxide to 90.2% for dothiepin-S-oxide.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Published under Short communication

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 5 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 20 Dec 2010, 13:57:27 EST