Simple microscale high-performance liquid chromatographic method for determination of furosemide in neonatal plasma

Sidhu, Jagdev S. and Charles, Bruce G. (1993) Simple microscale high-performance liquid chromatographic method for determination of furosemide in neonatal plasma. Journal of Chromatography - Biomedical Applications, 612 1: 161-165. doi:10.1016/0378-4347(93)80380-M


Author Sidhu, Jagdev S.
Charles, Bruce G.
Title Simple microscale high-performance liquid chromatographic method for determination of furosemide in neonatal plasma
Journal name Journal of Chromatography - Biomedical Applications
ISSN 0378-4347
Publication date 1993-01
Sub-type Other
DOI 10.1016/0378-4347(93)80380-M
Volume 612
Issue 1
Start page 161
End page 165
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract A simple microscale high-performance liquid chromatographic method using fluorescence detection for the quantitation of furosemide in neonatal plasma is described. Sample pre-treatment involved protein precipitation of 25 μl of plasma with 100 μl of acetonitrile. The mobile phase consisted of acetonitrile (460 ml) and 0.08 M orthophosphoric acid (540 ml) and was delivered at 1.1 ml/min. Calibration curves were linear from 0.1 to 25 μg/ml. Within-day and between-day imprecision (coefficient of variation) was 3.9-6.1, and 6.1-12.2%, respectively. Furosemide was eluted after 6.5 min and naproxen (internal standard) after 11.5 min. The assay was validated for application in neonatal plasma containing a wide range of albumin concentrations.
Keyword Infants
Urine
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Short communication

Document type: Journal Article
Sub-type: Other
Collection: School of Pharmacy Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 14 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 20 Dec 2010, 13:56:09 EST