Determination of dexamethasone in plasma of premature neonates using high-performance liquid chromatography

Schild, Paul Nicholas and Charles, Bruce Gordon (1994) Determination of dexamethasone in plasma of premature neonates using high-performance liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 658 1: 189-192. doi:10.1016/0378-4347(94)00192-8


Author Schild, Paul Nicholas
Charles, Bruce Gordon
Title Determination of dexamethasone in plasma of premature neonates using high-performance liquid chromatography
Journal name Journal of Chromatography B: Biomedical Sciences and Applications
ISSN 0378-4347
Publication date 1994-08-01
Sub-type Other
DOI 10.1016/0378-4347(94)00192-8
Volume 658
Issue 1
Start page 189
End page 192
Total pages 4
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
A high-performance liquid chromatographic assay is described for dexamethasone in neonatal plasma. Samples (100 μl) were extracted with ethyl acetate (1.0 ml) containing 50 ng of beclomethasone. The mobile phase of acetonitrile—0.01 M phosphate buffer (pH 7.0) (45:55, v/v) was pumped at a flow-rate of 1.0 ml/min through an Ultrasphere ODS column (5.0 μm particles). Detection was by UV absorbance at 240 nm. Calibration plots were linear (r2 > 0.999) from 10–1000 ng/ml. Within-day and between-day precision (C.V.%, coefficient of variation) between 15–900 ng/ml was 1.21–20.6%. Inaccuracy was 7.7%, or less. The minimum quantifiable concentration (C.V. ≈ 20%) was 15 ng/ml. Recovery (20–1000 ng/ml) exceeded 75%. Concomitant neonatal medication did not interfere.
Keyword Biological fluids
Trial
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Short communication

Document type: Journal Article
Sub-type: Other
Collection: School of Pharmacy Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 24 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 27 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 20 Dec 2010, 23:56:00 EST