Analysis of cisapride in neonatal plasma using high-performance liquid chromatography with a base-stable column and fluorescence detection

Preechagoon, Y. and Charles, B.G. (1995) Analysis of cisapride in neonatal plasma using high-performance liquid chromatography with a base-stable column and fluorescence detection. Journal of Chromatography B: Biomedical Sciences and Applications, 670 1: 139-143. doi:10.1016/0378-4347(95)00159-G


Author Preechagoon, Y.
Charles, B.G.
Title Analysis of cisapride in neonatal plasma using high-performance liquid chromatography with a base-stable column and fluorescence detection
Journal name Journal of Chromatography B: Biomedical Sciences and Applications
ISSN 0378-4347
Publication date 1995-08
Sub-type Article (original research)
DOI 10.1016/0378-4347(95)00159-G
Volume 670
Issue 1
Start page 139
End page 143
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
A simple, selective, sensitive and precise high-performance liquid chromatographic plasma assay for the prokinetic drug cisapride is described. Alkalinised samples of plasma (100 μl) were extracted with 1.0 ml of 10% (v/v) isopropanol in chloroform, dried, redissolved in mobile phase and injected. Chromatography was performed at 20°C by pumping a mobile phase of acetonitrile (370 ml) in pH 5.2, 0.02 M phosphate buffer (630 ml) at 1.0 ml/min through a C8 Symmetry column. Cisapride and the internal standard were detected by fluorescence monitoring at 295 nm (excitation) and 350 nm (emission), and were eluted 5 min and 8 min, respectively, after injection. Calibration plots in bovine serum albumin (3% w/v) were linear (r > 0.999) from 5 to 250 ng/ml. Intra-day and inter-day precision (C.V.) was 9.5%, or less, and the accuracy was within 5.5% of the nominal concentration over the range 8–200 ng/ml. Total assay recovery was above 82%. Endogenous plasma components, major cisapride metabolite (norcisapride), and other durgs used in neonatal pharmacotherapeutics did not interfere.
Keyword Prokinetic agent
Drug
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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