Measurement by HPLC of midazolam and major metabolite, 1-hydroxymidazolam in plasma of very premature neonates

Lee, Toong Chow and Charles, Bruce (1996) Measurement by HPLC of midazolam and major metabolite, 1-hydroxymidazolam in plasma of very premature neonates. Biomedical Chromatography, 10 2: 65-68. doi:10.1002/(SICI)1099-0801(199603)10:2<65::AID-BMC555>3.3.CO;2-H


Author Lee, Toong Chow
Charles, Bruce
Title Measurement by HPLC of midazolam and major metabolite, 1-hydroxymidazolam in plasma of very premature neonates
Journal name Biomedical Chromatography   Check publisher's open access policy
ISSN 0269-3879
1099-0801
Publication date 1996-03-01
Sub-type Article (original research)
DOI 10.1002/(SICI)1099-0801(199603)10:2<65::AID-BMC555>3.3.CO;2-H
Volume 10
Issue 2
Start page 65
End page 68
Total pages 4
Place of publication West Sussex,United Kingdom
Publisher John Wiley & Sons
Language eng
Formatted abstract
A simple, selective high-performance liquid chromatographic method with ultraviolet detection at 220 nm is described for quantitation of midazolam and its primary metabolite 1-hydroxymidazolam in 100 μL plasma samples from premature infants. A mobile phase of acetonitrile: tetrahydrofuran: phosphate buffer (0.01 M, pH 6.7) (35:5:60 v/v) was pumped at 1 ml/min through a C8 Symmetry® (150 x 3.9 mm) column. Plasma (100 μL) was extracted with 10% v/v isopropyl alcohol in dichloromethane containing 25 ng/mL climazolam (internal standard, IS) followed by back extraction into phosphoric acid (0.02 M). 1-Hydroxymidazolam, midazolam, and climazolam (IS) were eluted at 4.9, 7.4, and 8.4 min, respectively. Recoveries were > 70%. Calibration curves in blank plasma were linear (r > 0.999) from 12.5 to 800 ng/mL. Within-day and between-day imprecision (CV%) was 1.8-6.5%, and 4.1-8.8%, respectively. Inaccuracy was 12.3%. Application of the method was demonstrated by a pharmacokinetic analysis of midazolam and 1-hydroxymidazolam in plasma drawn from 15 premature neonates after a single intravenous dose of midazolam (0.1 mg/kg).
Keyword Performance liquid chromatography
Human serum
Pharmacokinetics
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Article first published online: 6 May 1999.

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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