Simple analysis of amoxycillin in plasma by high performance liquid chromatography with internal standardization and ultraviolet detection

Charles, Bruce and Chulavatnatol, Suvatna (1993) Simple analysis of amoxycillin in plasma by high performance liquid chromatography with internal standardization and ultraviolet detection. Biomedical Chromatography, 7 4: 204-207. doi:10.1002/bmc.1130070407


Author Charles, Bruce
Chulavatnatol, Suvatna
Title Simple analysis of amoxycillin in plasma by high performance liquid chromatography with internal standardization and ultraviolet detection
Journal name Biomedical Chromatography   Check publisher's open access policy
ISSN 0269-3879
1099-0801
Publication date 1993-07
Sub-type Article (original research)
DOI 10.1002/bmc.1130070407
Volume 7
Issue 4
Start page 204
End page 207
Total pages 4
Place of publication Bognor Regis, West Sussex, United Kingdom
Publisher John Wiley & Sons
Language eng
Formatted abstract
A simple high performance liquid chromatographic method with ultraviolet detection at 229 nm is described for quantitation of amoxycillin in plasma. After deproteination of plasma samples with perchloric acid and adjustment of the pH to 4.9, the supernatant was injected onto a reversed phase C18 column, using acetonitrile: phosphate buffer (0.01 M, pH 7.4) (1:25 v/v) as the mobile phase. Amoxycillin and the internal standard, cefadroxil, were eluted at 23 min and 12 min, respectively, without interference from endogenous substances. Processed samples were stable for at least 24 h at room temperature which permitted automated batch processing overnight. Calibration plots of the amoxycillin to cefadroxil peak-height ratio vs. amoxycillin concentration were linear (P < 0.0001; r⩾0.995) from 0.25 mg/L to at least 16.0 mg/L. Between-day and within-day imprecision (CV) ranged between 3.7% and 17.7%. Absolute recovery for amoxycillin and cefadroxil exceeded 82%. The application was demonstrated by the analysis of amoxycillin in human plasma after a single orall dose of amoxycillin (250 mg) suspension.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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Created: Mon, 20 Dec 2010, 13:51:00 EST