Rapid column liquid chromatographic analysis of flucloxacillin in plasma on a microparticulate pre-column

Charles, B.G., Foo, C.C. and Gath, J. (1994) Rapid column liquid chromatographic analysis of flucloxacillin in plasma on a microparticulate pre-column. Journal of Chromatography B: Biomedical Sciences and Applications, 660 1: 186-190. doi:10.1016/0378-4347(94)00255-X


Author Charles, B.G.
Foo, C.C.
Gath, J.
Title Rapid column liquid chromatographic analysis of flucloxacillin in plasma on a microparticulate pre-column
Journal name Journal of Chromatography B: Biomedical Sciences and Applications
ISSN 0378-4347
Publication date 1994-10
Sub-type Other
DOI 10.1016/0378-4347(94)00255-X
Volume 660
Issue 1
Start page 186
End page 190
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract A rapid, sensitive, accurate and precise high-performance liquid chromatographic assay is described for flucloxacillin in plasma. Samples (100 μl) containing dicloxacillin (internal standard) were extracted with ethyl acetate (2 ml). The mobile phase of acetonitrile (18%, v/v) in phosphate buffer (0.01 M, pH 7) was pumped at 1.2 ml/min through a 40 × 3.2 mm I.D. column (3 μm particles). Detection was at 220 nm. Calibration plots were linear (r > 0.9995) from 0.2 to 40 mg/l. Within-day and between-day coefficients of variation were less than 9% (n = 6). The detection limit was 0.05 mg/l and the limit of quantitation was 0.3 mg/l. Of 24 drugs tested, only phenytoin and carbamazepine may interfere in some patients' samples.
Keyword Isoxazolyl penicillins
Performance
Cloxacillin
Assay
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Short communication

Document type: Journal Article
Sub-type: Other
Collection: School of Pharmacy Publications
 
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Created: Mon, 20 Dec 2010, 13:48:55 EST