Zinc and DNA fragmentation in keratinocyte apoptosis: its inhibitory effect in UVB irradiated cells

Parat, Marie-Odile, Richard, Marie-Jeanne, Pollet, Ségolène, Hadjur, Christophe, Favier, Alain and Beani, Jean-Claude (1997) Zinc and DNA fragmentation in keratinocyte apoptosis: its inhibitory effect in UVB irradiated cells. Journal of Photochemistry and Photobiology B: Biology, 37 1-2: 101-106.


Author Parat, Marie-Odile
Richard, Marie-Jeanne
Pollet, Ségolène
Hadjur, Christophe
Favier, Alain
Beani, Jean-Claude
Title Zinc and DNA fragmentation in keratinocyte apoptosis: its inhibitory effect in UVB irradiated cells
Journal name Journal of Photochemistry and Photobiology B: Biology
Publication date 1997-01
Sub-type Article
DOI 10.1016/S1011-1344(96)07334-4
Volume number 37
Issue number 1-2
ISSN 1011-1344; 1873-2682
Start page 101
End page 106
Total pages 6
Place of publication Lausanne, Switzerland
Publisher Elsevier
Language eng
Formatted abstract Zinc has been shown to have antioxidant properties and to exhibit inhibitory effects on apoptosis. In this work we investigated the effect of zinc on DNA integrity and on apoptosis of HaCaT keratinocytes. Cells were submitted to zinc deprivation by a diffusible zinc chelator, (N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine) (TPEN) or supplied with zinc chloride and submitted to UVB radiation.
After cell exposure to TPEN for 2 h, strand breaks significantly impaired DNA resistance to alkaline denaturation. DNA strand breaks induced by a 6 h TPEN application were significantly prevented if zinc chloride was supplied together with the chelator. TPEN also generated, after 4–6 h of application, cytoplasmic histone-associated DNA fragments (mononucleosomes and oligonucleosomes), features of cell death by apoptosis.
Moreover, UVB irradiation led to early DNA strand breaks and to an increase in cytoplasmic nucleosomes which was maximum 10 h after irradiation. These effects were prevented by the supply of zinc chloride (0.1 mM) in the culture medium.
These results suggest that zinc ions interfere with the apoptosis process at an early stage, by decreasing DNA damage able to trigger apoptosis.
Keyword TPEN
Zinc
Keratinocyte
DNA
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article
Collection: School of Pharmacy Publications
 
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