Metal chelator NNN'N'-tetrakis-(2-pyridylmethyl)ethylene diamine inhibits the induction of heat shock protein 70 synthesis by heat in cultured keratinocytes

Parat, Marie-Odile, Richard, Marie-Jeanne, Favier, Alain and Beani, Jean-Claude (1998) Metal chelator NNN'N'-tetrakis-(2-pyridylmethyl)ethylene diamine inhibits the induction of heat shock protein 70 synthesis by heat in cultured keratinocytes. Biological Trace Element Research, 65 3: 261-270. doi:10.1007/BF02789101


Author Parat, Marie-Odile
Richard, Marie-Jeanne
Favier, Alain
Beani, Jean-Claude
Title Metal chelator NNN'N'-tetrakis-(2-pyridylmethyl)ethylene diamine inhibits the induction of heat shock protein 70 synthesis by heat in cultured keratinocytes
Formatted title
Metal chelator NNN'N'-tetrakis-(2-pyridylmethyl)ethylene diamine inhibits the induction of heat shock protein 70 synthesis by heat in cultured keratinocytes
Journal name Biological Trace Element Research   Check publisher's open access policy
ISSN 0163-4984
1559-0720
Publication date 1998-12
Sub-type Article (original research)
DOI 10.1007/BF02789101
Volume 65
Issue 3
Start page 261
End page 270
Total pages 10
Place of publication Totowa, NJ, United States
Publisher Humana Press
Language eng
Formatted abstract
Heat shock protein (HSP) synthesis results from various types of injury, including heat shock (HS) and some oxidants. The intracellular signals leading to HSP synthesis are not yet fully elucidated. We have studied the influence of NNN’N’-tetrakis(2-pyridylmethyl)ethylene diamine (TPEN), a metal chelator known to induce cellular zinc and copper deprivation, on resistance to heat and on hsp70 synthesis in HaCaT keratinocytes. TPEN was shown to sensitize HaCaT cells to heat shock. The effect of TPEN was neutralized by equimolar Zn2+. By the use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and Western blotting characterization of hsp70, it was shown that cultured HaCaT cells constitutively express the inducible form of hsp70. The application of TPEN alone slightly increases the level of hsp70 but inhibits its induction by HS. This inhibitory effect is related to metal deprivation, because it is eliminated when Cu2+ or Zn2+ ions are supplied together with TPEN. These results suggest that these metals are involved in the expression by keratinocytes of a stress protein which has a protective action against environmental stress.
Keyword Copper
Zinc
hsp70
TPEN
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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Created: Mon, 20 Dec 2010, 13:47:27 EST