Involvement of zinc in intracellular oxidant/antioxidant balance

Parat, Marie-Odile, Richard, Marie-Jeanne, Beani, Jean-Claude and Favier, Alain (1997) Involvement of zinc in intracellular oxidant/antioxidant balance. Biological Trace Element Research, 60 3: 187-204. doi:10.1007/BF02784439

Author Parat, Marie-Odile
Richard, Marie-Jeanne
Beani, Jean-Claude
Favier, Alain
Title Involvement of zinc in intracellular oxidant/antioxidant balance
Journal name Biological Trace Element Research   Check publisher's open access policy
ISSN 0163-4984
Publication date 1997-12
Sub-type Article (original research)
DOI 10.1007/BF02784439
Volume 60
Issue 3
Start page 187
End page 204
Total pages 18
Place of publication Totowa, NJ, United States
Publisher Humana Press
Language eng
Formatted abstract
The effect of zinc (Zn) on cellular oxidative metabolism is complex and could be explained by multiple complementary interactions. In this study, we evaluated the impact of Zn on the pro-oxidant/ antioxidant balance of HaCaT keratinocytes.
Cells were submitted to a diffusible metal chelator able to induce intracellular Zn deprivation, TPEN, in combination or not with Zn chloride (ZnCl2), in the culture medium. The intracellular amount of Zn, copper (Cu), and iron (Fe) was determined, as well as CuZnSOD and MnSOD activities and glutathione reserves. The consequence of the modulation of Zn concentration on lipid peroxidation was also evaluated.
TPEN induced a significant dose-dependent decrease in intracellular Zn and Cu (from 394–181 and 43–21 Μg/g protein, respectively, after 6 h of TPEN 50 ΜM). No significant change in intracellular Fe concentration was found following TPEN exposure. The SOD activities were unchanged after 6 h of TPEN 50 ΜM application, either CuZnSOD or MnSOD. Cells exposure to TPEN induced a deep time- and dosedependent decrease in their glutathione content (from 65–8 ΜM/g protein after 6 h of TPEN 50 ΜM), and a concomittant increase in glutathione in the cell-culture supernatants. No significant change in lipid peroxidation products was detected.
Keyword Antioxidant
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
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Created: Mon, 20 Dec 2010, 13:47:17 EST