Gill-associated virus and recombinant protein vaccination in Penaeus monodon

Underwood, Darren J., Cowley, Jeff A., Sellars, Melony J., Barnes, Andrew C., van Hulten, Marielle C. W. and Johnson, Karyn N. (2010) Gill-associated virus and recombinant protein vaccination in Penaeus monodon. Aquaculture, 308 3-4: 82-88. doi:10.1016/j.aquaculture.2010.08.027

Author Underwood, Darren J.
Cowley, Jeff A.
Sellars, Melony J.
Barnes, Andrew C.
van Hulten, Marielle C. W.
Johnson, Karyn N.
Title Gill-associated virus and recombinant protein vaccination in Penaeus monodon
Formatted title
Gill-associated virus and recombinant protein vaccination in Penaeus monodon
Journal name Aquaculture   Check publisher's open access policy
ISSN 0044-8486
Publication date 2010-10
Sub-type Article (original research)
DOI 10.1016/j.aquaculture.2010.08.027
Volume 308
Issue 3-4
Start page 82
End page 88
Total pages 7
Editor B. A. Costa-Pierce
E. M. Donaldson
G. Hulata
Place of publication Amsterdam, The Netherlands
Publisher Elsevier Scientific
Collection year 2011
Language eng
Formatted abstract
Recent studies in penaeid shrimp and various crustaceans showing specific antiviral responses to the DNA based White spot syndrome virus (WSSV) induced by pre-exposure to viral proteins are of particular research interest as invertebrates lack the classical adaptive immune response characterised in vertebrate animals. However, this protein-induced defence response has not been reported for any other invertebrate virus, and the mechanisms remain unknown. Here we investigated the generality of this defence response by challenging Penaeus monodon (Black Tiger shrimp) with the RNA based Gill-associated virus (GAV) following pre-exposure to fragments of the GAV gp116 envelope glycoprotein or to the p20 nucleocapsid protein. Shrimp were injected twice with recombinant GAV protein fragments expressed and purified from bacteria before challenge with GAV. Protein vaccination did not protect the shrimp, with mortality rates in shrimp injected with any of the GAV proteins not differing from shrimp either not injected with protein or injected with a non-specific protein. In addition, there was no significant difference in GAV RNA loads amongst the virus challenged shrimp groups. The data suggest that the efficacy of protection following protein-based vaccination may vary amongst different shrimp viruses.
© 2010.
Keyword Gill-associated virus
Black Tiger Shrimp (Penaeus monodon)
Invertebrate immunity
Envelope glycoproteins
Spot syndrome virus
Shrimp litopenaeus-vannamei
yellow head virus
C-type lectin
Antilipopolysaccharide factor
Molecular cloning
Envelope proteins
Viral diseases
Gav infection
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biological Sciences Publications
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Created: Fri, 17 Dec 2010, 11:54:21 EST by Gail Walter on behalf of School of Biological Sciences