Functional gene analysis suggests different acetogen populations in the Bovine Rumen and Tammar Wallaby Forestomach

Gagen, Emma J., Denman, Stuart E., Padmanabha, Jagadish, Zadbuke, Someshwar, Al Jassim, Rafat, Morrison, Mark and McSweeney, Christopher S. (2010) Functional gene analysis suggests different acetogen populations in the Bovine Rumen and Tammar Wallaby Forestomach. Applied and Environmental Microbiology, 76 23: 7785-7795. doi:10.1128/AEM.01679-10

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Author Gagen, Emma J.
Denman, Stuart E.
Padmanabha, Jagadish
Zadbuke, Someshwar
Al Jassim, Rafat
Morrison, Mark
McSweeney, Christopher S.
Title Functional gene analysis suggests different acetogen populations in the Bovine Rumen and Tammar Wallaby Forestomach
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
Publication date 2010-12
Sub-type Article (original research)
DOI 10.1128/AEM.01679-10
Open Access Status File (Publisher version)
Volume 76
Issue 23
Start page 7785
End page 7795
Total pages 11
Place of publication Washington, D.C., United States
Publisher American Society for Microbiology
Collection year 2011
Language eng
Formatted abstract
Reductive acetogenesis via the acetyl coenzyme A (acetyl-CoA) pathway is an alternative hydrogen sink to methanogenesis in the rumen. Functional gene-based analysis is the ideal approach for investigating organisms capable of this metabolism (acetogens). However, existing tools targeting the formyltetrahydrofolate synthetase gene (fhs) are compromised by lack of specificity due to the involvement of formyltetrahydrofolate synthetase (FTHFS) in other pathways. Acetyl-CoA synthase (ACS) is unique to the acetyl-CoA pathway and, in the present study, acetyl-CoA synthase genes (acsB) were recovered from a range of acetogens to facilitate the design of acsB-specific PCR primers. fhs and acsB libraries were used to examine acetogen diversity in the bovine rumen and forestomach of the tammar wallaby (Macropus eugenii), a native Australian marsupial demonstrating foregut fermentation analogous to rumen fermentation but resulting in lower methane emissions. Novel, deduced amino acid sequences of acsB and fhs affiliated with the Lachnospiraceae in both ecosystems and the Ruminococcaeae/Blautia group in the rumen. FTHFS sequences that probably originated from nonacetogens were identified by low "homoacetogen similarity" scores based on analysis of FTHFS residues, and comprised a large proportion of FTHFS sequences from the tammar wallaby forestomach. A diversity of FTHFS and ACS sequences in both ecosystems clustered between the Lachnospiraceae and Clostridiaceae acetogens but without close sequences from cultured isolates. These sequences probably originated from novel acetogens. The community structures of the acsB and fhs libraries from the rumen and the tammar wallaby forestomach were different (LIBSHUFF, P < 0.001), and these differences may have significance for overall hydrogenotrophy in both ecosystems.
Copyright © 2010,
Keyword Dehydrogenase/acetyl-coa synthase
Methane Production
Human Feces
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Received 14 July 2010/ Accepted 22 September 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
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Created: Sun, 12 Dec 2010, 00:12:40 EST