Far-infrared spectroscopy of protein higher-order structures

Falconer, Robert J., Zakaria, Hidayatul A., Fan, Yuan Y., Bradley, Andrew P. and Middelberg, Anton P.J. (2010) Far-infrared spectroscopy of protein higher-order structures. Applied Spectroscopy, 64 11: 1259-1264. doi:10.1366/000370210793335025

Author Falconer, Robert J.
Zakaria, Hidayatul A.
Fan, Yuan Y.
Bradley, Andrew P.
Middelberg, Anton P.J.
Title Far-infrared spectroscopy of protein higher-order structures
Journal name Applied Spectroscopy   Check publisher's open access policy
ISSN 0003-7028
Publication date 2010-11
Sub-type Article (original research)
DOI 10.1366/000370210793335025
Volume 64
Issue 11
Start page 1259
End page 1264
Total pages 6
Place of publication Plainfield, NJ, U.S.A.
Publisher Society for Applied Spectroscopy
Collection year 2011
Language eng
Abstract Far-infrared (FIR) spectroscopy in the spectral region of 50-450 cm -1 was used to study a series of protein higher-order structures constructed using β-lactoglobulin and Polyomavirus capsid protein VP1. There were marked differences in the spectra for β-lactoglobulin monomer and dimer and between untreated ß-lactoglobulin and heat-induced gels formed at neutral pH. Untreated ß-lactoglobulin and heat-induced gels formed at acidic pH exhibited little difference in their spectra. Assembly of the quaternary structure of Polyomavirus virus-like particles also caused large changes in the FIR spectra. These findings suggest that FIR spectroscopy may prove useful in studying some protein quaternary and higher-order structures. There was evidence of detection of β-lactoglobulin dimerization, intermolecular disulfide bonding in heat-induced neutral gels, and Polyomavirus virus-like particle assembly but no evidence that FIR could detect β-lactoglobulin fibrils with their polymeric structure and hydrogen-bonded intermolecular β-pleated sheeting. © 2010 Society for Applied Spectroscopy.
Keyword Terahertz
Vibrational spectrometry
Virus-like particle
Quaternary structure
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 12 Dec 2010, 00:12:37 EST