Sequence-Dependent Sorting of Recycling Proteins by Actin-Stabilized Endosomal Microdomains

Puthenveedu, MA, Lauffer, B, Temkin, P, Vistein, R, Carlton, P, Thorn, K, Taunton, J, Weiner, OD, Parton, RG and von Zastrow, M (2010) Sequence-Dependent Sorting of Recycling Proteins by Actin-Stabilized Endosomal Microdomains. Cell, 143 5: 761-773. doi:10.1016/j.cell.2010.10.003

Author Puthenveedu, MA
Lauffer, B
Temkin, P
Vistein, R
Carlton, P
Thorn, K
Taunton, J
Weiner, OD
Parton, RG
von Zastrow, M
Title Sequence-Dependent Sorting of Recycling Proteins by Actin-Stabilized Endosomal Microdomains
Journal name Cell   Check publisher's open access policy
ISSN 0092-8674
Publication date 2010-11
Sub-type Article (original research)
DOI 10.1016/j.cell.2010.10.003
Volume 143
Issue 5
Start page 761
End page 773
Total pages 13
Place of publication Cambridge, MA, United States
Publisher Cell Press
Collection year 2011
Language eng
Abstract The functional consequences of signaling receptor endocytosis are determined by the endosomal sorting of receptors between degradation and recycling pathways. How receptors recycle efficiently, in a sequence-dependent manner that is distinct from bulk membrane recycling, is not known. Here, in live cells, we visualize the sorting of a prototypical sequence-dependent recycling receptor, the beta-2 adrenergic receptor, from bulk recycling proteins and the degrading delta-opioid receptor. Our results reveal a remarkable diversity in recycling routes at the level of individual endosomes, and indicate that sequence-dependent recycling is an active process mediated by distinct endosomal subdomains distinct from those mediating bulk recycling. We identify a specialized subset of tubular microdomains on endosomes, stabilized by a highly localized but dynamic actin machinery, that mediate this sorting, and provide evidence that these actin-stabilized domains provide the physical basis for a two-step kinetic and affinity-based model for protein sorting into the sequence-dependent recycling pathway. © 2010 Elsevier Inc.
Keyword Clathrin-mediated Endocytosis
Arp2/3 Complex
Dynamic actin
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 112 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 118 times in Scopus Article | Citations
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Created: Sun, 12 Dec 2010, 00:11:01 EST