DNA vaccine delivery by densely-packed and short microprojection arrays to skin protects against vaginal HSV-2 challenge

Kask, Angela Shaulov, Chen, Xianfeng, Marshak, Joshua O., Dong, Lichun, Saracino, Misty, Chen, Dexiang, Jarrahian, Courtney, Kendall, Mark A. and Koelle, David M. (2010) DNA vaccine delivery by densely-packed and short microprojection arrays to skin protects against vaginal HSV-2 challenge. Vaccine, 28 47: 7483-7491. doi:10.1016/j.vaccine.2010.09.014


Author Kask, Angela Shaulov
Chen, Xianfeng
Marshak, Joshua O.
Dong, Lichun
Saracino, Misty
Chen, Dexiang
Jarrahian, Courtney
Kendall, Mark A.
Koelle, David M.
Title DNA vaccine delivery by densely-packed and short microprojection arrays to skin protects against vaginal HSV-2 challenge
Journal name Vaccine   Check publisher's open access policy
ISSN 0264-410X
1873-2518
1355-2732
1359-5938
Publication date 2010-11-03
Sub-type Article (original research)
DOI 10.1016/j.vaccine.2010.09.014
Volume 28
Issue 47
Start page 7483
End page 7491
Total pages 9
Place of publication Oxford, U.K.
Publisher Elsevier
Collection year 2011
Language eng
Formatted abstract
There is an unmet medical need for a prophylactic vaccine against herpes simplex virus (HSV). DNA vaccines and cutaneous vaccination have been tried for many applications, but few reports combine this vaccine composition and administration route. We compared DNA administration using the NanopatchTM, a solid microprojection device coated with vaccine comprised of thousands of short (110m) densly-packed projections (70m spacing), to standard intramuscular DNA vaccination in a mouse model of vaginal HSV-2 infection. A dose-response relationship was established for immunogenicity and survival in both vaccination routes. Appropriate doses administered by NanopatchTM were highly immunogenic and enabled mouse survival. Vaginal HSV-2 DNA copy number day 1 post challenge correlated with survival, indicating that vaccine-elicited acquired immune responses can act quickly and locally. Solid, short, densely-packed arrays of microprojections applied to the skin are thus a promising route of administration for DNA vaccines. 
© 2010 Elsevier B.V.

Keyword DNA vaccine
Microprojection
Herpes simplex virus
Skin
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Sun, 12 Dec 2010, 00:00:14 EST