PTRF-cavin -1 expression decreases the migration of PC3 prostate cancer cells: Role of matrix metalloprotease 9

Cho, Sanda Aung, Hill, Michelle M., Bastiani, Michele, Parton, Robert G. and Parat, Marie-Odile (2011) PTRF-cavin -1 expression decreases the migration of PC3 prostate cancer cells: Role of matrix metalloprotease 9. European Journal of Cell Biology, 90 2-3: 136-142. doi:10.1016/j.ejcb.2010.06.004


Author Cho, Sanda Aung
Hill, Michelle M.
Bastiani, Michele
Parton, Robert G.
Parat, Marie-Odile
Title PTRF-cavin -1 expression decreases the migration of PC3 prostate cancer cells: Role of matrix metalloprotease 9
Journal name European Journal of Cell Biology   Check publisher's open access policy
ISSN 0171-9335
1618-1298
0724-5130
Publication date 2011-02
Year available 2010
Sub-type Article (original research)
DOI 10.1016/j.ejcb.2010.06.004
Volume 90
Issue 2-3
Start page 136
End page 142
Total pages 7
Place of publication Jena, Germany
Publisher Urban und Fischer Verlag
Collection year 2011
Language eng
Formatted abstract
Caveolae are specialized plasma membrane subdomains with a distinct lipid and protein composition, which play an essential role in cell physiology by performing trafficking and signalling functions. The structure and functions of caveolae have been shown to require caveolin-1, a major protein component of caveolae. Caveolin-1 expression and secretion are increased in metastatic prostate cancer, and caveolin-1 seems to contribute to prostate cancer growth and metastasis. Recently, a cytoplasmic protein named PTRF (Polymerase I and Transcript Release Factor) or cavin-1 was found to be required, in concert with caveolin-1, for the formation and functions of caveolae. Genetic ablation of PTRF results in loss of caveolae while caveolin-1 is still expressed, albeit at reduced level, but associates with flat plasma membrane. In metastatic PC3 prostate cancer cells that express abundant caveolin-1 but no PTRF, heterologous PTRF expression restores caveola formation and caveolin-1 distribution (; Cell 132, 113-124). We now show that PTRF/cavin-1-expressing PC3 cells exhibit decreased migration, and that this effect is mediated by reduced MMP9 production. PTRF/cavin-1, and to a lesser extent, cavin-2, -3, and -4 all decreased MMP9. We further show that the PTRF/cavin-1-mediated reduction of MMP9 production is independent of caveola formation. Taken together, our results suggest that PTRF/cavin-1 expression alters prostate cancer aggressiveness. © 2010 Elsevier GmbH. All rights reserved.

Keyword Caveolae
PTRF
Cavin
MMP9
Prostate cancer
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 21 August 2010

 
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Created: Wed, 01 Dec 2010, 16:09:34 EST by Charna Kovacevic on behalf of School of Pharmacy