Genetic changes in cell lines from human malignant mesothelioma during long term culturing

Relan, V., Quinn, K. R., Wright, C. M., Savarimuthu, S. M., Tan, M. E., Clarke, B. E., Duhig, E. E., Yang, I. A., Fong, K. M. and Bowman, R. V. (2010). Genetic changes in cell lines from human malignant mesothelioma during long term culturing. In: Special Issue: Abstracts of the Thoracic Society of Australia & New Zealand (TSANZ) and the Australian & New Zealand Society of Respiratory Science (ANZSRS) 2010 Annual Scientific Meetings. Brisbane 2010: The Joint TSANZ & ANZSRS Annual Scientific Meetings, Brisbane, QLD, Australia, (A74-A74). 19–24 March 2010. doi:10.1111/j.1440-1843.2010.01736.x


Author Relan, V.
Quinn, K. R.
Wright, C. M.
Savarimuthu, S. M.
Tan, M. E.
Clarke, B. E.
Duhig, E. E.
Yang, I. A.
Fong, K. M.
Bowman, R. V.
Title of paper Genetic changes in cell lines from human malignant mesothelioma during long term culturing
Conference name Brisbane 2010: The Joint TSANZ & ANZSRS Annual Scientific Meetings
Conference location Brisbane, QLD, Australia
Conference dates 19–24 March 2010
Proceedings title Special Issue: Abstracts of the Thoracic Society of Australia & New Zealand (TSANZ) and the Australian & New Zealand Society of Respiratory Science (ANZSRS) 2010 Annual Scientific Meetings   Check publisher's open access policy
Journal name Respirology   Check publisher's open access policy
Place of Publication Richmond, VIC, Australia
Publisher Wiley-Blackwell Publishing Asia
Publication Year 2010
Sub-type Published abstract
DOI 10.1111/j.1440-1843.2010.01736.x
ISSN 1323-7799
1440-1843
Volume 15
Issue Supp. 1
Start page A74
End page A74
Total pages 1
Collection year 2012
Language eng
Formatted Abstract/Summary
Aim: This study aimed to assess genetic changes during long term culturing of two malignant mesothelioma (MM) cell lines (MM04 and MM05).

Results and Discussion: Spindle shaped cells were observed in both passages for both cell lines. TEM also confirmed the same morphology in early and late passages in MM05. Doubling time experiments confirmed faster growth of late passage versus early passage cultures. ((MM05 (P9) 45.49 hours vs. (P47) 43.28 hours). Doubling time experiments for MM04 late passage is underway (The result will be presented at the conference). T Test (p < 0.001) on gene expression profile displayed down regulation of 8 genes (TMEM165, GREB1, HS566426, PRUNE, SLC10A7, C4ORF38, TBN, USP53) and up regulation of 6 genes (TMEM2, CDKAL1, TIAM2, RPL23A, STAT3, UPK1B) were found to be in late passages (figure). The genetic imbalances occurring in early to late culture steps reflected the tendency of MM cells toward genetic changes during long term culturing.
© 2010 The Author(s)
Journal Compilation © 2010 Asian Pacific Society of Respirology
Subjects 11 Medical and Health Sciences
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Presented during the Thoracic Society of Australia & New Zealand (TSANZ)'s Combined Poster Session "OELD/Population Health SIG - Primary Care SIG" as Poster TP140.

Document type: Conference Paper
Collection: School of Medicine Publications
 
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Created: Tue, 30 Nov 2010, 14:37:00 EST by Dr Vandana Relan on behalf of Medicine - Prince Charles Hospital