Assessing intratumor distribution and uptake with MBBG versus MIBG imaging and targeting xenografted PC12-pheochromocytoma cell line

Clerc, Jérôme, Mardon, Karine, Galons, Hervé, Loc'h, Christian, Lumbroso, Jean, Merlet, Pascal, Zhu, Jiarong, Jeusset, Josette, Syrota, André and Fragu, Philippe (1995) Assessing intratumor distribution and uptake with MBBG versus MIBG imaging and targeting xenografted PC12-pheochromocytoma cell line. Journal of Nuclear Medicine, 36 5: 859-866.

Author Clerc, Jérôme
Mardon, Karine
Galons, Hervé
Loc'h, Christian
Lumbroso, Jean
Merlet, Pascal
Zhu, Jiarong
Jeusset, Josette
Syrota, André
Fragu, Philippe
Title Assessing intratumor distribution and uptake with MBBG versus MIBG imaging and targeting xenografted PC12-pheochromocytoma cell line
Journal name Journal of Nuclear Medicine   Check publisher's open access policy
ISSN 0161-5505
1535-5667
Publication date 1995-05-01
Sub-type Article (original research)
Volume 36
Issue 5
Start page 859
End page 866
Total pages 8
Place of publication Reston, VA, United States
Publisher Society of Nuclear Medicine
Language eng
Abstract The heterogeneity of tumor uptake is likely to substantially limit the effectiveness of metaiodobenzylguanidine (MIBG) therapy. This study was done to establish whether metabromobenzyl-guanidine (MBBG) can target neuroendocrine tumors and to provide intratumor biodistribution and uptake information in comparison to MIBG. Methods: MBBG and MIBG tumor uptake and kinetic studies were performed in experimental PC-12 pheochromocytoma grown in nude mice. Intratumor distribution studies were performed using autoradiography and secondary ion mass spectrometry (SIMS) microscopy, because the latter technique can detect and potentially quantify both drugs concomitantly within the same tumor specimen. Results: MBBG uptake in PC12 tumors was early (2 hr) and intense (80% ID/g). Retention values were similar for both drugs 24 hr postinjection. At the cellular level, MBBG mostly accumulated in the cytosol. At the multicellular level, cells exhibited staining, but in many areas, SIMS images of both drugs were not spatially correlated. Conclusion: MBBG targeted experimental pheochromocytoma efficiently with high early uptake values. Bromine-76-MBBG is a promising means of imaging and quantifying tumor uptake with PET. Both drugs were localized in the cytosol, but the correlation between the two distributions, as assessed by the values of the standardized local concentrations, was weak although significant multicellularly.
Keyword Metabolite radiotherapy
Metabromobenzylguanidine
Metaiodobenzylguanidine
Pheochromocytoma
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Advanced Imaging Publications
 
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Created: Fri, 26 Nov 2010, 22:33:33 EST by Dr Karine Mardon on behalf of Centre for Advanced Imaging