Prohepcidin localises to the Golgi compartment and secretory pathway in hepatocytes

Wallace, Daniel F., Summerville, Lesa, Lusby, Patricia E. and Subramaniam, V. Nathan (2005) Prohepcidin localises to the Golgi compartment and secretory pathway in hepatocytes. Journal of Hepatology, 43 4: 720-728. doi:10.1016/j.jhep.2005.02.047


Author Wallace, Daniel F.
Summerville, Lesa
Lusby, Patricia E.
Subramaniam, V. Nathan
Title Prohepcidin localises to the Golgi compartment and secretory pathway in hepatocytes
Journal name Journal of Hepatology   Check publisher's open access policy
ISSN 0168-8278
1600-0641
Publication date 2005-10
Sub-type Article (original research)
DOI 10.1016/j.jhep.2005.02.047
Volume 43
Issue 4
Start page 720
End page 728
Total pages 9
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract
Background/Aims
Hepcidin is a liver-expressed peptide which plays an important role in the regulation of iron metabolism. It is a negative regulator of iron absorption and release of iron from cells. The aims of this study were to analyse the expression and localisation of prohepcidin in liver and cell lines.
Methods
We generated antibodies against recombinant mouse prohepcidin and studied its expression in cell lines, primary hepatocytes and livers of normal mice and mice with abnormalities in iron metabolism.
Results
Prohepcidin localised to the secretory pathway, primarily the Golgi apparatus in liver cells and tissues. Hfe and β2-microglobulin knockout mice have similar levels of prohepcidin protein expression as compared to wild-type mice despite increased iron stores. Sex-linked anaemia mice have iron deficiency and no prohepcidin expression in the liver.
Conclusions
Prohepcidin protein is present in the secretory pathway of liver cells. Despite iron loading, mouse models of haemochromatosis have comparatively normal levels of prohepcidin expression whereas mice with iron deficiency have no prohepcidin expression.
Keyword Regulatory hormone
Iron homeostasis
Iron overload
Subcellular localization
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Thu, 25 Nov 2010, 10:33:20 EST