Synergistic effects of ascorbic acid and thiazolidinedione on secretion of high molecular weight adiponectin from human adipocytes

Rose, F. J., Webster, J., Barry, J. B., Phillips, L. K., Richards, A. A. and Whitehead, J. P. (2010) Synergistic effects of ascorbic acid and thiazolidinedione on secretion of high molecular weight adiponectin from human adipocytes. Diabetes Obesity and Metabolism, 12 12: 1084-1089. doi:10.1111/j.1463-1326.2010.01297.x


Author Rose, F. J.
Webster, J.
Barry, J. B.
Phillips, L. K.
Richards, A. A.
Whitehead, J. P.
Title Synergistic effects of ascorbic acid and thiazolidinedione on secretion of high molecular weight adiponectin from human adipocytes
Journal name Diabetes Obesity and Metabolism   Check publisher's open access policy
ISSN 1462-8902
1463-1326
Publication date 2010-12-01
Sub-type Article (original research)
DOI 10.1111/j.1463-1326.2010.01297.x
Volume 12
Issue 12
Start page 1084
End page 1089
Total pages 6
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2011
Language eng
Formatted abstract
Aim: To test the hypothesis that ascorbic acid (AA) and thiazolidinedione (TZD) would have additive effects on HMW adiponectin secretion by virtue of different modes of action.
Methods: We determined the effects of supplementation of AA and/or TZD on expression and secretion of total and HMW adiponectin from human Simpson-Golabi-Behmel syndrome (SGBS) adipocytes in the absence or presence of the proinflammatory cytokine TNFα.
Results: AA supplementation significantly increased secretion of HMW adiponectin (1.7-fold) without altering adiponectin expression or total adiponectin secretion. TZD significantly increased expression (3-fold) and secretion of total (1.4-fold) but not HMW adiponectin. Combined supplementation resulted in a significant increase in expression (3-fold) and secretion of total (1.8-fold) and HMW (5-fold) adiponectin. Similar results were seen in cells co-treated with TNFα.
Conclusions: These data show that AA and TZD have synergistic rather than simple additive effects on secretion of HMW adiponectin from human adipocytes and raise the possibility that differences in AA levels may contribute to the variability in adiponectin multimer profiles and efficacy of TZD in humans. Our results also provide a rationale for longitudinal clinical trials investigating the effects of AA supplementation with or without TZD on adiponectin and metabolic profiles. © 2010 Blackwell Publishing Ltd.
Keyword Adipokine
Diabetes
Post-translational modification
Obesity
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
UQ Diamantina Institute Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 21 Nov 2010, 10:05:58 EST