Ovalbumin-sensitized mice are good models for airway hyperresponsiveness but not acute physiological responses to allergen inhalation

Zosky, G. R., Larcombe, A. N., White, O. J., Burchell, J. T., Janosi, T. Z.., Hantos, Z., Holt, P. G., Sly, P. D. and Turner, D. J. (2008) Ovalbumin-sensitized mice are good models for airway hyperresponsiveness but not acute physiological responses to allergen inhalation. Clinical And Experimental Allergy, 38 5: 829-838. doi:10.1111/j.1365-2222.2007.02884.x


Author Zosky, G. R.
Larcombe, A. N.
White, O. J.
Burchell, J. T.
Janosi, T. Z..
Hantos, Z.
Holt, P. G.
Sly, P. D.
Turner, D. J.
Title Ovalbumin-sensitized mice are good models for airway hyperresponsiveness but not acute physiological responses to allergen inhalation
Journal name Clinical And Experimental Allergy   Check publisher's open access policy
ISSN 0954-7894
1365-2222
Publication date 2008-05
Sub-type Article (original research)
DOI 10.1111/j.1365-2222.2007.02884.x
Volume 38
Issue 5
Start page 829
End page 838
Total pages 10
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell
Language eng
Abstract Background: Asthma is a chronic inflammatory disease that is characterized clinically by airway hyperresponsiveness (AHR) to bronchoconstricting agents. The physiological response of the asthmatic lung to inhaled allergen is often characterized by two distinct phases: an early-phase response (EPR) within the first hour following exposure that subsides and a late-phase response (LPR) that is more prolonged and may occur several hours later. Mouse models of asthma have become increasingly popular and should be designed to exhibit an EPR, LPR and AHR. Objective: To determine whether a common model of asthma is capable of demonstrating an EPR, LPR and AHR. Methods: BALB/c mice were sensitized to ovalbumin (OVA) and challenged with one or three OVA aerosols. Changes in lung mechanics in response to allergen inhalation were assessed using a modification of the low-frequency forced oscillation technique (LFOT). In order to assess AHR, changes in lung mechanics in response to aerosolized methacholine were assessed using LFOT. Inflammatory cell infiltration into the lung was measured via bronchoalveolar lavage (BAL). ELISAs were used to measure inflammatory cytokines in the BAL and levels of IgE in the serum. Results: An EPR was only detectable after three OVA aerosols in approximately half of the mice studied. There was no evidence of an LPR despite a clear increase in cellular infiltration 6 h post-allergen challenge. AHR was present after a single OVA aerosol but not after three OVA aerosols. Conclusions: The lack of an LPR, limited EPR and the absence of a link between the LPR and AHR highlight the limitations of this mouse model as a complete model of the lung dysfunction associated with asthma.
Keyword airway hyperresponsiveness
asthma
early-phase response
late-phase response
mouse model
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Faculty of Health and Behavioural Sciences -- Publications
 
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Created: Wed, 17 Nov 2010, 12:00:04 EST