Assay for urinary desmosines in a healthy pre-pubertal population using an improved extraction technique

Winfield, Kaye R., Gard, Samantha, Kent, G. Neil, Sly, Peter D. and Brennan, Siobhain (2006) Assay for urinary desmosines in a healthy pre-pubertal population using an improved extraction technique. Annals of Clinical Biochemistry, 43 2: 146-152. doi:10.1258/000456306776021571


Author Winfield, Kaye R.
Gard, Samantha
Kent, G. Neil
Sly, Peter D.
Brennan, Siobhain
Title Assay for urinary desmosines in a healthy pre-pubertal population using an improved extraction technique
Journal name Annals of Clinical Biochemistry   Check publisher's open access policy
ISSN 0004-5632
1758-1001
Publication date 2006-03
Sub-type Article (original research)
DOI 10.1258/000456306776021571
Volume 43
Issue 2
Start page 146
End page 152
Total pages 7
Place of publication London, United Kingdom
Publisher Royal Society of Medicine Press
Language eng
Abstract Background: Current evidence indicates that increased desmosine excretion reflects the active inflammatory status of some connective tissue diseases. Our goal was to establish a reliable method of detection and to investigate the normal distribution of urinary desmosine excretion in a healthy pre-pubertal population. Method: Urine was collected from healthy volunteers aged four weeks to 12 years old. We modified a published high-performance liquid chromatography (HPLC) method by (a) increasing hydrolysis time and temperature and (b) increasing cellulose column size. Results: Our modified method had small inter- and intra-assay variability, with coefficients of variation of <6.4% and 5.3%, respectively. There was positive correlation between isodesmosine and desmosine (r2=0.91). There was no significant diurnal or day-to-day variability in total desmosine levels. A reference range for healthy pre-pubertal children aged four weeks to 12 years was established. Conclusion: The modified HPLC method is reliable with low variability. The technique can now be applied as a non-invasive research or diagnostic tool for children with chronic lung disease.
Keyword Obstructive Pulmonary-disease
Collagen degaradation products
Cystic-fibrosis
Elastin
Lung
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Faculty of Health and Behavioural Sciences -- Publications
 
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Created: Wed, 17 Nov 2010, 11:58:59 EST