Thymic indoleamine 2,3-dioxygenase-positive eosinophils in young children: Potential role in maturation of the naive immune system

Tulic, Meri K., Sly, Peter D., Andrews, David, Crook, Maxine, Davoine, Francis, Odemuyiwa, Solomon O., Charles, Adrian, Hodder, Megan L., Prescott, Susan L., Holt, Patrick G. and Moqbel, Redwan (2009) Thymic indoleamine 2,3-dioxygenase-positive eosinophils in young children: Potential role in maturation of the naive immune system. American Journal of Pathology, 175 5: 2043-2052. doi:10.2353/ajpath.2009.090015


Author Tulic, Meri K.
Sly, Peter D.
Andrews, David
Crook, Maxine
Davoine, Francis
Odemuyiwa, Solomon O.
Charles, Adrian
Hodder, Megan L.
Prescott, Susan L.
Holt, Patrick G.
Moqbel, Redwan
Title Thymic indoleamine 2,3-dioxygenase-positive eosinophils in young children: Potential role in maturation of the naive immune system
Journal name American Journal of Pathology   Check publisher's open access policy
ISSN 0002-9440
1525-2191
Publication date 2009-11
Sub-type Article (original research)
DOI 10.2353/ajpath.2009.090015
Volume 175
Issue 5
Start page 2043
End page 2052
Total pages 10
Place of publication Bethesda, MD, United States
Publisher American Society for Investigative Pathology
Language eng
Abstract Eosinophils expressing indoleamine 2, 3-dioxygenase (IDO) may contribute to T-helper cell (Th)2 predominance. To characterize human thymus IDO+ eosinophil ontogeny relative to Th2 regulatory gene expression, we processed surgically obtained thymi from 22 children (age: 7 days to 12 years) for immunohistochemistry and molecular analysis, and measured cytokine and kynurenine levels in tissue homogenates. Luna+ eosinophils (∼2% of total thymic cells) decreased in number with age (P = 0.02) and were IDO+. Thymic IDO immunoreactivity (P = 0.01) and kynurenine concentration (P = 0.01) decreased with age as well. In addition, constitutively-expressed interleukin (IL)-5 and IL-13 in thymus supernatants was highest in youngest children. Eosinophil numbers correlated positively with expression of the Th2 cytokines IL-5, IL-13 (r = 0.44, P = 0.002), and IL-4 (r = 0.46, P = 0.005), transcription factor signal transducer and activator of transcription-6 (r = 0.68, P = 0.001), and the chemokine receptor, CCR3 (r = 0.17, P = 0.04), but negatively with IL-17 mRNA (r = -0.57, P = 0.02) and toll-like receptor 4 expression (r = -0.74, P = 0.002). Taken together, these results suggest that functional thymic IDO+ eosinophils during human infant life may have an immunomodulatory role in Th2 immune responses.
Keyword Dendritic Cells
T-cells
Cutting Edge
Differentiation
Expression
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Faculty of Health and Behavioural Sciences -- Publications
 
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Created: Wed, 17 Nov 2010, 11:54:44 EST