Suppression of the asthmatic phenotype by ultraviolet B-induced, antigen-specific regulatory cells

McGlade, J. P., Gorman, S., Zosky, G. R., Larcombe, A. N., Sly, P. D., Finlay-Jones, J. J., Turner, D. J. and Hart, P. H. (2007) Suppression of the asthmatic phenotype by ultraviolet B-induced, antigen-specific regulatory cells. Clinical And Experimental Allergy, 37 9: 1267-1276. doi:10.1111/j.1365-2222.2007.02750.x


Author McGlade, J. P.
Gorman, S.
Zosky, G. R.
Larcombe, A. N.
Sly, P. D.
Finlay-Jones, J. J.
Turner, D. J.
Hart, P. H.
Title Suppression of the asthmatic phenotype by ultraviolet B-induced, antigen-specific regulatory cells
Journal name Clinical And Experimental Allergy   Check publisher's open access policy
ISSN 0954-7894
1365-2222
Publication date 2007-09
Sub-type Article (original research)
DOI 10.1111/j.1365-2222.2007.02750.x
Volume 37
Issue 9
Start page 1267
End page 1276
Total pages 10
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell
Language eng
Abstract Background: Over recent decades, there has been a significant global increase in the prevalence of asthma, an inflammatory disease of the respiratory system. While ultraviolet radiation (UV) has been used successfully in the treatment of inflammatory conditions such as psoriasis, studies of UV-induced regulation of allergic respiratory responses have been rare, and have not analysed in vivo measurements of airway hyperresponsiveness (AHR) or the antigen specificity of the UV-induced effects. Objective: To investigate the regulatory properties of erythemal ultraviolet B (UVB) irradiation of the skin and the induction of allergen-induced airway immunity in a murine asthma model, and to examine the mechanisms involved. Methods: BALB/c mice were exposed to a single erythemal dose of UV 3 days before intraperitonial sensitization (day 0) and boost (day 14) with the antigen, ovalbumin (OVA). Airway-associated, asthma-like responses to aerosolized OVA at day 21 were analysed including (a) AHR measured in vivo, (b) OVA-specific proliferative responses and cytokine production by cells from the lung-draining lymph nodes (LDLN), and (c) inflammatory cells and cytokines in the bronchoalveolar lavage fluid. To determine UVB-induced mechanisms of regulation, LDLN cells from UVB irradiated, OVA-sensitized mice were adoptively transferred into naïve BALB/c mice that were subsequently sensitized and challenged with OVA, or a non-specific antigen. Results: UVB irradiation of skin significantly suppressed AHR to methacholine and OVA-specific responses in the LDLN and in the lung compartment. Reduced OVA-specific responses by LDLN cells from both UVB irradiated mice and mice that received 5 × 106 LDLN cells from UVB irradiated, but not from non-irradiated, OVA-sensitized mice suggested that UVB-induced regulatory cells are responsible for many of the asthma-reducing effects of dorsal UVB exposure. Conclusion: UVB irradiation of skin suppresses AHR and cellular responses of the airways to respiratory allergens. Further, this study implicates UVB or its downstream mediators as a potential approach to reducing the severity of asthma.
Keyword asthma
immune regulation
regulatory cells
respiratory
ultraviolet
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Faculty of Health and Behavioural Sciences -- Publications
 
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