Dose and time response after intraamniotic endotoxin in preterm lambs

Kramer, B.W., Moss, T.J., Willet, K.E., Newnham, J.P., Sly, P.D., Kallapur, S.G., Ikegami, M. and Jobe, A.H. (2001) Dose and time response after intraamniotic endotoxin in preterm lambs. American Journal of Respiratory and Critical Care Medicine, 164 6: 982-988.

Author Kramer, B.W.
Moss, T.J.
Willet, K.E.
Newnham, J.P.
Sly, P.D.
Kallapur, S.G.
Ikegami, M.
Jobe, A.H.
Title Dose and time response after intraamniotic endotoxin in preterm lambs
Journal name American Journal of Respiratory and Critical Care Medicine   Check publisher's open access policy
ISSN 1073-449X
Publication date 2001-09
Sub-type Article (original research)
Volume 164
Issue 6
Start page 982
End page 988
Total pages 7
Place of publication New York, NY, United States
Publisher American Thoracic Society
Language eng
Abstract Intraamniotic endotoxin causes chorioamnionitis, which is followed by improved fetal lung function after 4 d in fetal sheep. We evaluated 0.1 mg, 1 mg, 4 mg, and 10 mg endotoxin for inflammation and lung maturation effects after 7 d. Four and 10 mg endotoxin caused similar lung maturation and inflammation in the lung and chorioamnion. The number of neutrophils in cord blood and the inflammatory cells in alveolar lavage and fetal lung tissue increased in a dose-dependent manner. Lower endotoxin doses induced indicators of chorioamnionitis, lung and systemic inflammation without inducing lung maturation. Therefore, some degree of inflammation can occur without subsequent lung maturation. The inflammatory changes caused by 4 mg endotoxin were assessed after 5 h, 24 h, 72 h, and 7 d to discern local versus systemic inflammation after intraamniotic endotoxin. At 5 h active inflammatory cells were in the airways producing hydrogen peroxide, and interleukin-6 and -8 were increased in the cord blood indicating both lung and systemic responses. Cells recruited into the amniotic fluid produced proinflammatory cytokine mRNA for 7 d with no cytokine mRNA in chorioamnion, lung, or spleen after 72 h. The cells in the amniotic fluid may be a source of prolonged fetal exposure to proinflammatory cytokines.
Keyword Respiratory distress syndrome
Bronchopulmonary dysplasia
Neutrophil recruitment
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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Created: Wed, 17 Nov 2010, 11:35:35 EST